Anti-ZMYM3 抗体 (ab19165)
Key features and details
- Rabbit polyclonal to ZMYM3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZMYM3 antibody
ZMYM3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZMYM3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide: K PEVVDSTE SI PVSDEDS DAM VDDPND EDFV PFRPR RSPRM SLRS SVSQRA, corresponding to amino acids 250-300 of Human ZMYM3
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ポジティブ･コントロール
- Extract from HeLa cell extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 0.0968% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified using an epitope specific to ZNF261 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 152 kDa).
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| IHC-P |
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/10000 - 1/25000. Detects a band of approximately 200 kDa (predicted molecular weight: 152 kDa). |
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IHC-P
1/500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Most abundant in brain, moderate in muscle and heart, low in other tissues except placenta. -
関連疾患
Note=A chromosomal aberration involving ZMYM3 may be a cause of X-linked mental retardation in Xq13.1. Translocation t(X;13)(q13.1;?). -
配列類似性
Contains 9 MYM-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 9203 Human
- Entrez Gene: 56364 Mouse
- Entrez Gene: 317260 Rat
- Omim: 300061 Human
- SwissProt: Q14202 Human
- SwissProt: Q9JLM4 Mouse
- Unigene: 522684 Human
- Unigene: 23458 Mouse
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製品の状態
There are 2 isoforms produced by alternative splicing. -
別名
- DXS6673E antibody
- KIAA0385 antibody
- MYM antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZMYM3 antibody (ab19165)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma tissue labelling ZMYM3 with ab19165 at 1/5000 (0.2µg/ml). Detection: DAB.
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Western blot - Anti-ZMYM3 antibody (ab19165)Ab19165 at 0.1 ug/ml staining ZNF261 in 10 ug of Nuclear extract from HeLa cells by Western blot. Detection was by chemiluminescence with an exposure time of 15 minutes. Ab19165 at 0.1 ug/ml staining ZMYM3 in 10 ug of Nuclear extract from HeLa cells by Western blot. Detection was by chemiluminescence with an exposure time of 15 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab19165 は 2 報の論文で使用されています。
- Acquaviva L et al. Ensuring meiotic DNA break formation in the mouse pseudoautosomal region. Nature 582:426-431 (2020). PubMed: 32461690
- La HM et al. GILZ-dependent modulation of mTORC1 regulates spermatogonial maintenance. Development 145:N/A (2018). PubMed: 30126904