Zinc Quantification Kit (Fluorometric) (ab176725)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell culture extracts, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 13 ng/ml
製品の概要
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製品名
Zinc Quantification Kit (Fluorometric)
Zinc キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts -
アッセイタイプ
Quantitative -
検出感度
>= 13 ng/ml -
種交差性
交差種: Mammals, Other species -
製品の概要
Abcam's Zinc Quantification Kit (Fluorometric) (ab176725) provides a robust method for detecting zinc concentration in biological samples using our proprietary Zn Detector, in which Zinc binds to the probe with enhanced fluorescence at Ex/Em= 485/525 nm. The Zinc probe exhibits a large increase in fluorescence in response to Zn2+ (greater than 200~300 folds). It has high Zn2+ -specificity with little responses to other metals, including Ca2+, Mg2+, Mn2+, and Cu2+. The assay can be used with biological samples such as serum, plasma, and urine with detection sensitivity at 0.2 µM (13 ng/mL).
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特記事項
Zinc is an essential trace mineral element that plays an important role in a number of biological processes. It is an essential factor required for many enzymes, protein structures, and control of genetic expression. Zinc status also affects basic processes of cell division, growth, differentiation, development, and aging. Clinical signs of zinc deficiency include acrodermatitis, low immunity, diarrhea, poor healing, stunting, hypogonadism, fetal growth failure, teratology and abortion. Simple, direct and automation-ready procedures for measuring zinc ion are highly desirable in research and drug discovery.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 200 tests Assay Buffer 1 x 15ml Zn Detector 1 vial ZnCl2 Standard 1 vial -
研究分野
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関連性
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. -
別名
- Zn
- Zn++
- Zn2+
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab176725 は 7 報の論文で使用されています。
- Olivares-González L et al. NUTRARET: Effect of 2-Year Nutraceutical Supplementation on Redox Status and Visual Function of Patients With Retinitis Pigmentosa: A Randomized, Double-Blind, Placebo-Controlled Trial. Front Nutr 9:847910 (2022). PubMed: 35387197
- Braakhuis A et al. A Modern Flexitarian Dietary Intervention Incorporating Web-Based Nutrition Education in Healthy Young Adults: Protocol for a Randomized Controlled Trial. JMIR Res Protoc 10:e30909 (2021). PubMed: 34931994
- Maremanda KP et al. Zinc deficient diet exacerbates the testicular and epididymal damage in type 2 diabetic rat: Studies on oxidative stress-related mechanisms. Reprod Biol 20:191-201 (2020). PubMed: 32245730
- Kim JM et al. Impaired Cu-Zn Superoxide Dismutase (SOD1) and Calcineurin (Cn) Interaction in ALS: A Presumed Consequence for TDP-43 and Zinc Aggregation in Tg SOD1G93A Rodent Spinal Cord Tissue. Neurochem Res 44:228-233 (2019). PubMed: 29299811
- Guillory RJ et al. Preclinical In-Vivo Evaluation and Screening of Zinc Based Degradable Metals for Endovascular Stents. JOM (1989) 71:1436-1446 (2019). PubMed: 33731979
- Reinhardt S et al. Identification of disulfiram as a secretase-modulating compound with beneficial effects on Alzheimer's disease hallmarks. Sci Rep 8:1329 (2018). Mouse . PubMed: 29358714
- Li C et al. PINK1 and PARK2 Suppress Pancreatic Tumorigenesis through Control of Mitochondrial Iron-Mediated Immunometabolism. Dev Cell 46:441-455.e8 (2018). PubMed: 30100261