Zinc Assay Kit (ab102507)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 15 min
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 µg/ml
製品の概要
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製品名
Zinc Assay Kit
Zinc キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Quantitative -
検出感度
1 µg/ml -
全工程の試験時間
0h 15m -
製品の概要
Zinc Assay Kit ab102507 is a convenient colorimetric assay in which Zinc binds to a ligand with development of absorbance at 560 nm.
The zinc assay can be used with biological samples such as serum, plasma, csf or urine with detection sensitivity 0.2 µg/ml (~1-3 µM).
Zinc assay protocl summary:
- add samples and standards to wells
- add reaction mix and incubate for 10 min
- analyze with a microplate reader -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K387 Zinc Colorimetric Assay Kit. K387-100 is the same size as the 100 test size of ab102507.
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of 2+. Zinc is an essential mineral of great biological significance, because many enzymes require it as an essential cofactor.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 100 tests 7% TCA 1 x 5ml Zinc Reagent 1 1 x 16ml Zinc Reagent 2 1 x 4ml Zinc Standard 1 x 0.1ml -
研究分野
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関連性
Zinc, a metallic chemical element, symbol Zn and atomic number 30 is chemically similar to Magnesium due to its similar size and sole oxidation state of +2. Zinc is an essential mineral of great biological significance since many enzymes require it as an essential cofactor. Examples of zinc’s biological roles include signal transduction, gene expression, regulation of apoptosis, synaptic plasticity and prostate gland function. -
別名
- Zn
- Zn++
- Zn2+
画像
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Indicated strains were cultured in low zinc medium (SD0, pH ~4.7), exposed to 25 µM ZnSO4 and zinc acquisition determined at indicated time points by measuring how much zinc remained in the cell free supernatant. C. albicans wild type acquires all measurable zinc within 60 minute; zrt2Δ does not; complementation restored zinc acquisition to 68%. Experiment performed three times
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Zinc measured in cell lysates showing quantity (nmol) per 1 mln of tested cells
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Zinc measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample
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Standard curve (colourimetric) : mean of duplicates (+/- SD) with background substracted
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Representative Standard Curve using ab102507.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab102507 は 13 報の論文で使用されています。
- Gaffke L et al. Impaired ion homeostasis as a possible associate factor in mucopolysaccharidosis pathogenesis: transcriptomic, cellular and animal studies. Metab Brain Dis 37:299-310 (2022). PubMed: 34928474
- Murata K et al. Clinical Significance of Serum Zinc Levels on the Development of Sarcopenia in Cirrhotic Patients. Cancer Diagn Progn 2:184-193 (2022). PubMed: 35399181
- Dera HA et al. Melatonin attenuates cerebral hypoperfusion-induced hippocampal damage and memory deficits in rats by suppressing TRPM7 channels. Saudi J Biol Sci 29:2958-2968 (2022). PubMed: 35531206
- Tuong ZK et al. Resolving the immune landscape of human prostate at a single-cell level in health and cancer. Cell Rep 37:110132 (2021). PubMed: 34936871
- Xu W et al. Hypozincemia in COVID-19 Patients Correlates With Stronger Antibody Response. Front Immunol 12:785599 (2021). PubMed: 35058926