Anti-ZFR 抗体 (ab272572)

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Western blot - Anti-ZFR antibody (ab272572)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFR antibody (ab272572)
  • Immunocytochemistry/ Immunofluorescence - Anti-ZFR antibody (ab272572)

Key features and details

  • Rabbit polyclonal to ZFR
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-ZFR antibody

  • 製品の詳細

    Rabbit polyclonal to ZFR

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human ZFR aa 450-600.
    Database link: Q96KR1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: K562 cell lysate. IHC-P: Human pancreas tissue. ICC/IF: U-251 MG.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
WB
Human
アプリケーション Abreviews 特記事項
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
特記事項
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
WB
Use a concentration of 0.04 - 0.4 µg/ml.

ターゲット情報

  • 機能

    Involved in postimplantation and gastrulation stages of development. Involved in the nucleocytoplasmic shuttling of STAU2. Binds to DNA and RNA.

  • 組織特異性

    Expressed in lung, liver, lymphocytes, heart, pancreas, placenta, brain and kidney.

  • 配列類似性

    Contains 1 DZF domain.

  • 細胞内局在

    Nucleus. Cytoplasm. Cytoplasmic granule. Chromosome. Associated with chromosome foci in meiotic cells. Localizes in somatodendritic compartment of primary hippocampal neurons. Colocalizes with STAU2 in several cytosolic RNA granules.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ41312 antibody
      • hZFR antibody
      • M phase phosphoprotein homolog antibody
      • M-phase phosphoprotein homolog antibody
      • zfr antibody
      • ZFR_HUMAN antibody
      • zinc finger RNA binding protein antibody
      • Zinc finger RNA-binding protein antibody
      see all

    画像

    • Western blot - Anti-ZFR antibody (ab272572)
      Western blot - Anti-ZFR antibody (ab272572)
      Anti-ZFR antibody (ab272572) at 0.04 µg/ml + K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFR antibody (ab272572)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZFR antibody (ab272572)

      Paraffin-embedded formalin-fixed Human pancreas tissue stained for ZFR using ab272572 at 1/50 in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-ZFR antibody (ab272572)
      Immunocytochemistry/ Immunofluorescence - Anti-ZFR antibody (ab272572)

      PFA fixed, Triton X-100 permeablized human cell line U-251 MG labeling ZFR (green) using ab272572 at 2 ug/ml in ICC/IF analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272572 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272572 は論文での使用が確認できていません。

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