Anti-Zfp281/ZNF281 抗体 (ab101318)
Key features and details
- Rabbit polyclonal to Zfp281/ZNF281
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-Zfp281/ZNF281 antibody -
製品の詳細
Rabbit polyclonal to Zfp281/ZNF281 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Rabbit, Horse, Dog, Human, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human Zfp281/ZNF281 aa 800 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab130905) -
ポジティブ・コントロール
- This antibody gave a positive signal in a wild type mouse embryonic stem cell lysate, which is absent from a Zfp281/ZNF281 null mouse embryonic stem cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101318の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
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IP | (1) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa). |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin and ornithine decarboxylase. Binds to the G-rich box in the enhancer region of these genes. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23528 Human
- Entrez Gene: 226442 Mouse
- Entrez Gene: 305083 Rat
- SwissProt: Q9Y2X9 Human
- Unigene: 59757 Human
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別名
- GC box binding zinc finger protein 1 antibody
- GC-box-binding zinc finger protein 1 antibody
- GZP1 antibody
see all
画像
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All lanes : Anti-Zfp281/ZNF281 antibody (ab101318) at 1 µg/ml
Lane 1 : wild type mouse embryonic stem cell lysate
Lane 2 : Zfp281/ZNF281 null mouse embryonic stem cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab101318 は 10 報の論文で使用されています。
- LeBlanc L et al. β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation. iScience 25:103541 (2022). PubMed: 34977504
- Laudadio I et al. ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis. Int J Mol Sci 23:N/A (2022). PubMed: 36142169
- Nicolai S et al. ZNF281/Zfp281 is a target of miR-1 and counteracts muscle differentiation. Mol Oncol 14:294-308 (2020). PubMed: 31782884
- Xian Z et al. lncRNA UCA1 Contributes to 5-Fluorouracil Resistance of Colorectal Cancer Cells Through miR-23b-3p/ZNF281 Axis. Onco Targets Ther 13:7571-7583 (2020). PubMed: 32801774
- Guo J et al. The transcription factor Zfp281 sustains CD4+ T lymphocyte activation through directly repressing Ctla-4 transcription. Cell Mol Immunol N/A:N/A (2019). PubMed: 31511645
- Ishiuchi T et al. Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development. Cell Rep 27:1742-1754.e6 (2019). PubMed: 31067460
- Huang X et al. Zfp281 is essential for mouse epiblast maturation through transcriptional and epigenetic control of Nodal signaling. Elife 6:N/A (2017). PubMed: 29168693
- Fidalgo M et al. Zfp281 Coordinates Opposing Functions of Tet1 and Tet2 in Pluripotent States. Cell Stem Cell 19:355-69 (2016). PubMed: 27345836
- Pieraccioli M et al. ZNF281 contributes to the DNA damage response by controlling the expression of XRCC2 and XRCC4. Oncogene 35:2592-601 (2016). PubMed: 26300006
- Fidalgo M et al. Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming. Proc Natl Acad Sci U S A 109:16202-7 (2012). WB, ChIP, IP . PubMed: 22988117