Anti-ZCWCC1 抗体 (ab14429)
Key features and details
- Rabbit polyclonal to ZCWCC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ZCWCC1 antibody -
製品の詳細
Rabbit polyclonal to ZCWCC1 -
由来種
Rabbit -
特異性
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
-
免疫原
Synthetic peptide corresponding to Human ZCWCC1 aa 600-700. The epitope recognised by ab14429 maps to a region of human zinc finger, CW-type with coiled-coil domain 1.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified using an epitope specific to ZCWCC1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14429の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use a concentration of 3 µg/ml.
|
|
| WB |
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 118 kDa.
|
| 特記事項 |
|---|
|
IP
Use a concentration of 3 µg/ml. |
|
WB
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 118 kDa. |
ターゲット情報
-
機能
Exhibits a cytosolic function in lipogenesis, adipogenic differentiation, and lipid homeostasis by increasing the activity of ACLY, possibly preventing its dephosphorylation. May act as a transcriptional repressor. Down-regulates CA9 expression. -
組織特異性
Highly expressed in smooth muscle, pancreas and testis. -
配列類似性
Contains 1 CW-type zinc finger. -
細胞内局在
Nucleus. Cytoplasm, cytosol. Mainly located in the nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 22880 Human
- SwissProt: Q9Y6X9 Human
- Unigene: 555918 Human
-
別名
- AC004542.C22.1. antibody
- CW type with coiled coil domain 1 antibody
- KIAA0852 antibody
see all
画像
-
Western blot - Anti-ZCWCC1 antibody (ab14429)All lanes : Anti-ZCWCC1 antibody (ab14429) at 0.1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 118 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-ZCWCC1 antibody (ab14429)Immunoprecipitation of ZCWCC1 with ab14429 at 3 µg/mL.
Lane 1: ab14429 + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.Lane 2: IgG control + Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Detection: Chemiluminescence
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab14429 は論文での使用が確認できていません。