Anti-ZC3H8 抗体 (ab97821)
Key features and details
- Rabbit polyclonal to ZC3H8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZC3H8 antibody -
製品の詳細
Rabbit polyclonal to ZC3H8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 31-264 of ZC3H8 (NP_115883)
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ポジティブ･コントロール
- 293T; A431; H1299; HeLaS3; HepG2; MOLT4 or Raji lysates NCIN87 xenograft (IHC-P) HeLa cells (IF)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97821の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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| IHC-P |
Use at an assay dependent concentration.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 84524 Human
- SwissProt: Q8N5P1 Human
- Unigene: 418416 Human
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別名
- Fliz1 antibody
- Zc3h8 antibody
- ZC3H8_HUMAN antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-ZC3H8 antibody (ab97821)Immunofluorescence analysis of paraformaldehyde fixed HeLa, using ab97821 at 1:200 dilution. Lower image is merged with DNA probe.
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Western blot - Anti-ZC3H8 antibody (ab97821)All lanes : Anti-ZC3H8 antibody (ab97821) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Raji whole cell lysate
Predicted band size: 34 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H8 antibody (ab97821)Immunohistochemical analysis of paraffin embedded NCIN87 xenograft, using ab97821 at 1:500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97821 は 2 報の論文で使用されています。
- Takahashi H et al. The role of Mediator and Little Elongation Complex in transcription termination. Nat Commun 11:1063 (2020). PubMed: 32102997
- Egloff S et al. The 7SK snRNP associates with the little elongation complex to promote snRNA gene expression. EMBO J 36:934-948 (2017). PubMed: 28254838