Anti-ZC3H12D 抗体 (ab100862)
Key features and details
- Rabbit polyclonal to ZC3H12D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-ZC3H12D antibody -
製品の詳細
Rabbit polyclonal to ZC3H12D -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
A 16 amino acid peptide from the intermediate residues of Human ZC3H12D protein (NP_997243).
-
ポジティブ･コントロール
- WB: Fetal small intestine lysate and fetal spleen lystae IHC-P: Fetal small intestine
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Lyophilized:Add 200 ul of sterile distilled water -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100862の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 58 kDa.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 58 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
May regulate cell growth likely by suppressing RB1 phosphorylation (PubMed:19531561). May function as RNase and regulate the levels of target RNA species (Potential). In association with ZC3H12A enhances the degradation of interleukin IL-6 mRNA level in activated macrophages (PubMed:26134560). Serve as a tumor suppressor in certain leukemia cells (PubMed:17210687). Overexpression inhibits the G1 to S phase progression through suppression of RB1 phosphorylation (PubMed:19531561). -
組織特異性
Expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines. -
関連疾患
A chromosomal aberration involving ZC3H12D may be the cause of the transformation of follicular lymphoma (FL) to diffuse large B-cell lymphoma (DLBCL). Translocation t(2;6)(p12;q25) with IGK. Resulting protein may not be expressed. -
配列類似性
Belongs to the ZC3H12 family.
Contains 1 C3H1-type zinc finger. -
細胞内局在
Cytoplasm. Nucleus; Cytoplasm. Localized as discrete granules. Colocalized with mRNA-processing body markers, AGO2 and DCP1A, but not with a stress granule maker, TIA1, in the cytoplasm and Cytoplasm, P-body. Colocalizes with ZC3H12A in GW bodies (GWBs) (PubMed:26134560). - Information by UniProt
-
参照データベース
- Entrez Gene: 340152 Human
- Omim: 611106 Human
- SwissProt: A2A288 Human
- Unigene: 632618 Human
-
別名
- C6orf95 antibody
- ZC3H12D antibody
- FLJ00361 antibody
see all
画像
-
Western blot - Anti-ZC3H12D antibody (ab100862)All lanes : Anti-ZC3H12D antibody (ab100862) at 1/500 dilution
Lane 1 : Human fetal small intestine lysate
Lane 2 : Human fetal spleen lysate
Predicted band size: 58 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H12D antibody (ab100862)ab100862, at a 1/100 dilution, staining ZC3H12D in formalin-fixed, paraffin-embedded Human fetal small intestine tissue, showing cytoplasmic staining, by Immunohistochemistry.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab100862 は 1 報の論文で使用されています。
- Huang S et al. The putative tumor suppressor Zc3h12d modulates toll-like receptor signaling in macrophages. Cell Signal : (2011). IHC-P ; Mouse . PubMed: 22036805