製品名Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor
製品の詳細Cell permeable caspase-3 inhibitor
- Caspase-3 Inhibitor
- Z-DEVD-FMK (cell permeable)
生理活性の詳細A potent, cell-permeable, and irreversible inhibitor of caspase-3. Also inhibits caspase-6, caspase-7, caspase-8, and caspase-10. Once in the cell, endogenous esterase activity hydrolyzes the methyl groups to form the biologically active form. Therefore, when using with isolated, purified or recombinant caspase enzymes, pre-treatment with an esterase is required.
配列DEVD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Asp-1 = Asp(OMe); Glu-2 = Glu(OMe); Asp-4 = Asp(OMe))
保存方法Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解性Soluble in DMSO to 20 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
HeLa cells were incubated at 37 °C for 1h with vehicle control (0 µM) and different concentrations of Z-D(OMe)E(OMe)VD(OMe)-FMK (ab120488). After this incubation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLA cells correlates with an increase in Z-D(OMe)E(OMe)VD(OMe)-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120488 は 2 報の論文で使用されています。
- De D et al. Ethanolic extract of leaf of Dillenia pentagyna reduces in-vitro cell migration and induces intrinsic pathway of apoptosis via downregulation of NF-?ß in human NSCLC A549 cells. J Cell Biochem 120:19841-19857 (2019). PubMed: 31318086
- Yang H et al. Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways. World J Gastrointest Oncol 11:377-392 (2019). PubMed: 31139308