アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    z-dna-antibody-ab2079.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling DNA / RNA DNA / Nucleotides
Share by email

Anti-z DNA 抗体 (ab2079)

  • Datasheet
Reviews (1)Q&A (6)References (4)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Sheep polyclonal to z DNA
  • Suitable for: IP, In situ hybridization
  • Reacts with: Species independent
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-DNAJA2 antibody [EPR11302(B)] (ab157216)
一次抗体
Product image
Anti-ds DNA antibody [35I9 DNA] (ab27156)
二次抗体
Product image
Rabbit Anti-Sheep IgG H&L (HRP) (ab6747)

関連製品

製品の概要

  • 製品名

    Anti-z DNA antibody
  • 製品の詳細

    Sheep polyclonal to z DNA
  • 由来種

    Sheep
  • アプリケーション

    適用あり: IP, In situ hybridizationmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Z-DNA

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.50
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

関連製品

  • Isotype control

    • Sheep IgG - Isotype Control (ab37385)

アプリケーション

Our Abpromise guarantee covers the use of ab2079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent dilution.
In situ hybridization Use at an assay dependent dilution. See below; Cerna, A. et al, European journal of Histochemistry.

ターゲット情報

  • 関連性

    Z-DNA is one of the many possible double helical structures of DNA. It is a left-handed double helical structure in which the double helix winds to the left in a zig-zag pattern (instead of to the right, like the more common B-DNA form). Z-DNA is thought to be one of three biologically active double helical structures along with A- and B-DNA.

プロトコール

  • Immunoprecipitation protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
  • 参考文献 (4)

    ab2079 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2079 は 4 報の論文で使用されています。

    • Leonova K  et al. TRAIN (Transcription of Repeats Activates INterferon) in response to chromatin destabilization induced by small molecules in mammalian cells. Elife 7:N/A (2018). PubMed: 29400649
    • Safina A  et al. FACT is a sensor of DNA torsional stress in eukaryotic cells. Nucleic Acids Res 45:1925-1945 (2017). PubMed: 28082391
    • Lu P  et al. The Za domain of fish PKZ converts DNA hairpin with d(GC)n inserts to Z-conformation. Acta Biochim Biophys Sin (Shanghai) 45:1062-8 (2013). PubMed: 24113090
    • Cerná A  et al. Z-DNA, a new in situ marker for transcription. Eur J Histochem 48:49-56 (2004). PubMed: 15145775

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-z DNA antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Testis)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 18 2020

    Question

    Thanks for your reply.
    I appreciate that information regarding the antibody is proprietary, I am not seeking disclosure of their methods. However, I still need to know if they carried out any testing on the antigen to establish its purity. In other words, can the supplier state that only Z-DNA was injected?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 13 2012

    Answer

    Having checked with the lab, we can confirm that a preparation of Z-DNA (i.e. left-handed DNA) of sufficient purity has been injected.
    I am sorry that we are unable to provide more details, but I hope this information is nevertheless helpful to you. We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Apr 13 2012

    Question

    Please provide details on the method and the quality control used in the synthesis of the brominated DNA used to raise this antibody. Is the starting material removed from the bromination product prior to further use? Are DNA breakdown products removed from the bromination products? Is the bromination done under controlled conditions? What temperature is the DNA bromination carried out under?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2012

    Answer

    Thank you for contacting us and your patience.
    Unfortunately, the antigen used to produce this product was made a few years ago in a laboratory at a private university. Most information regarding this antibody is propriety, however, I can tell you the following: Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen.
    I hope this information is still helpful for you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 20 2012

    Question

    I have purchased Z-DNA antibody(ab2079) and have two questions: - What is the immunogen for this antibody - Are you aware of a DNA sequence that could be used as a positive control for immuprecipitation Many thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2011

    Answer

    Thank you for your inquiry. The lab where this antibody is produced informed me that, unfortunately, at this time they do not have a specific DNA sequence that could be used as a positive control for immunoprecipitation. We will update the on-line datasheet for this product as soon as we receive additional data. We apologize for any inconvenience this may cause.

    Read More

    Abcam Scientific Support

    Answered on Sep 23 2011

    Question

    I have purchased Z-DNA antibody(ab2079) and have two questions: - What is the immunogen for this antibody - Are you aware of a DNA sequence that could be used as a positive control for immuprecipitation Many thanks in advance

    Read More

    Abcam community

    Verified customer

    Asked on Sep 19 2011

    Answer

    Thank you for contacting us. Z-DNA antibody was raised in sheep using brominated poly-(DG-DC)-left handed DNA (Z-DNA) complexed to methylated BSA as the immunogen. I asked the lab about a recommended positive control. I will contact you again as soon as I receive this information.

    Read More

    Abcam Scientific Support

    Answered on Sep 19 2011

    Question

    I have purchased Z-DNA antibody(ab2079), and then crosslinked it to Z-DNA using formaldehyde. Would you give me some suggestuions about how to shear the cross-linked DNA to 500-1000 base pairs ? By the way, the sonication treatment is not allowed in the current situation.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 27 2006

    Answer

    Thank you for your enquiry. Your question is indeed an interesting one. The two most commonly used techniques for the preparation of chromatin are sonication and a mild micrococcal nuclease digestion. They can both be employed for use in cross linked chromatin. However, the nuclease digestion of chromatin relies on the fact that the digestion of DNA is obscured by the association of the DNA with the nucleosome template and therefore the chromatin is preferentially digested into mono, di- and tri-nucleosomes etc. However, given that you are digesting free Z-DNA this is not applicable as either MNase (or DNAse I or II) will fully digest the DNA to completion. The only thing that will provide you with suitable resolution is a restriction enzyme. This has been adopted in the context of chromatin in the following publication using a mild PstI digestion; Kang SH, Vieira K, Bungert J. 2002 Combining chromatin immunoprecipitation and DNA footprinting: a novel method to analyze protein-DNA interactions in vivo. Nucleic Acids Res. May 15;30(10) I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Jan 02 2007

    Question

    I have ordered the Z-DNA antibody(ab2079),but I don't know the effective final concentration.Do you know the binding plateau of the antibody? Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 25 2006

    Answer

    Thank you for your enquiry. There is a reference on the datasheet for this antibody(ab2079) which as far as I can tell recommends a dilution of 1:10 for ISH. European Journal of Histochemistry. 2004 48 (1) 49-56. Z-DNA a new in situ marker for transcription. I hope this reference will be helpful. If you are using the antibody for IP, then I would recommend using 10-500 µg cell lysate plus 0.5-5 µg of antibody. The precise amount will need to be optimised for your particular application. I hope this is helpful. Please do not hesitate to contact us again if you require more assistance.

    Read More

    Abcam Scientific Support

    Answered on Aug 25 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.