Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 32°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 32°C
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: FabuLight secondary Fab #111-587-008
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
In the case of our application, no nuclei staining was observed. Instead, strong signals in a part of neurite structures were observed.
Abcam response
Thank you for submitting this abreview. We are sorry to hear that you have problems with this antibody. This antibody do not guarantee IHC-Fr. We will welcome more data or suggestion from our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Etsuo Susaki
Verified customer
投稿 Nov 30 2018