Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate antigen repair solution
Permeabilization
Yes - PBST (0.5% Triton X-100 in PBS)
Specification
testis
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in PBST (0.1% tween in PBS)
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alex Fluor 555 donkey anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Additional data
Additional Notes
This anti-YTHDC2 antibody is very specific for spermatogonia in mouse testis.
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投稿 Aug 30 2019