Anti-Yes1 抗体 (ab106158)
Key features and details
- Rabbit polyclonal to Yes1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Yes1 antibody
Yes1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Yes1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Cow, Macaque monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human Yes1 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab127968) -
ポジティブ･コントロール
- This antibody gave a positive signal in the following whole cell lysates: HepG2; Jurkat; HEK293; Caco2; MCF7.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106158の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
ターゲット情報
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機能
Promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm > cytosol. In epithelial cells infected with Neisseria gonorrhoeae, forms aggregates beneath bacterial microcolonies. - Information by UniProt
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参照データベース
- Entrez Gene: 507632 Cow
- Entrez Gene: 7525 Human
- Entrez Gene: 22612 Mouse
- Entrez Gene: 24884 Rat
- Omim: 164880 Human
- SwissProt: P07947 Human
- SwissProt: Q04736 Mouse
- SwissProt: F1LM93 Rat
see all -
製品の状態
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), SRC (P12931), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
別名
- c Yes antibody
- Cellular Yes 1 protein antibody
- Cellular Yes1 protein antibody
see all
画像
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Western blot - Anti-Yes1 antibody (ab106158)All lanes : Anti-Yes1 antibody (ab106158) at 1 µg/ml (Milk Blocked - 3%)
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 27 kDa, 42 kDa, 57 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
データシートおよび資料
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Datasheet download
参考文献 (0)
ab106158 は論文での使用が確認できていません。
