Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A2780 whole cell lysate 2 µg/µl 100 µl)
Total protein in input
200 µg
Specification
A2780 whole cell lysate 2 µg/µl 100 µl
Immuno-precipitation step
Protein A/G
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Western Blot antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Additional data
Additional Notes
lane 1: use yb1 antibody
lane 2: use another antibody as negative control
lane 2: use another antibody as negative control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 08 2008