Application
Western blot
Sample
Human Cell lysate - whole cell (Human Renal Proximal Tubular cells)
Loading amount
10 µg
Specification
Human Renal Proximal Tubular cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1 % Tween-20, 1 % BSA in TBS
Secondary antibody
Name
Non-Abcam antibody was used: DakoCytomation
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 and 36 kDa Non-specific: none kDa
Additional data
Additional Notes
Believe the 50 kDa band to represent the whole YB-1 molecule and the 36 kDa band is a fragment with the C-terminal portion removed by 20S proteosome.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Robert Jenkins
Verified customer
投稿 Apr 30 2009