製品の概要

  • 製品名
    Y-27632 dihydrochloride
  • 製品の詳細
    Selective Rho kinase inhibitor
  • 生理活性の詳細
    Also available in simple stock solutions (ab144494) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 精製度
    > 99%

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

ab120129 has not yet been referenced specifically in any publications.

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Question
Answer



Y-27632 dihydrochloride, ab120129, is cell-permeable.

Read More

Question

Dear xxxx,

could you please inform me about the lead time, if I send you my purchase order for 3x10mg today - and what is the longest shelf life of this product, which you’ve currently available on stock?

Thank you very much

Best regards
















Dijana Stojaković
Purchasing

________________________

PAA Laboratories GmbH
Now Part of GE Healthcare Life Sciences

PAA-Strasse 1
4061 Pasching, Austria
Tel.: +43 7229 64865 - 313
Fax: +43 7229 64866
blocked::http://www.paa.com/

blocked::http://www.gelifesciences.com/paa

Registered office: Pasching

Regional court: Linz FN 87291w

VAT-No.: ATU 44001307









Von: orders@abcam.com [mailto:orders@abcam.com]
Gesendet: Donnerstag, 11. Oktober 2012 17:14
An: Stojakovic, Dijana (GE Healthcare)
Betreff: Reply from Abcam to your enquiry re: WG: Proforma Invoice 46200 from Abcam [CCE4238739]



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Dijana



Thank you for contacting us.



We only produce the sizes we sent you in the proforma.



If you require further assistance please do not hesitate to contact us.


Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=4238739


Best regards,
Lina

Lina Joseph
Multilingual Customer Service Assistant
Abcam plc
https://www.abcam.com

* Por favor no dude en contactarme en castellano si lo prefiere


Your original inquiry to Abcam:






Dear ,

Thank you very much for your quotation.



Would you be so kind and let me know, if the pack size 200mg is also available?







Thank you





Best regards





Dijana Stojaković
Purchasing

________________________

PAA Laboratories GmbH
Now Part of GE Healthcare Life Sciences

PAA-Strasse 1
4061 Pasching, Austria
Tel.: +43 7229 64865 - 313
Fax: +43 7229 64866
blocked::http://www.paa.com/

blocked::http://www.gelifesciences.com/paa

Registered office: Pasching

Regional court: Linz FN 87291w

VAT-No.: ATU 44001307









Von: mailto:orders@abcam.com mailto:[mailto:orders@abcam.com]
Gesendet: Donnerstag, 11. Oktober 2012 16:28
An: Stojakovic, Dijana (GE Healthcare)
Betreff: Proforma Invoice 46200 from Abcam



















https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Dijana

Many thanks for your enquiry.

Please find attached your Proforma Invoice as requested.

Should you have any further questions please reply to this email or contact us on +44 (0)1223 696000.

If you decide to place an order for these items, please quote Proforma Invoice number 46200.

We look forward to hearing from you.



[CCE4238189]














Discover more at abcam.com


























Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE4238739]













Discover more at abcam.com

Read More
Answer

Thank you for your reply. My colleague has passed on your enquire to me and I hope to be able to answer your questions.

The compound is currently in stock and therefore if you were to place your order before 4 pm today it should be with you on Monday the 15th of October.

All the stock we have will be guaranteed for its stability for up to 6 months following purchase providing it is stored as is stated on the product vial and the vial is kept tightly sealed. It may be that the product is stable for considerably longer than this but we do not have any stability data to support this and cannot therefore guarantee it.

When preparing the compound for use we would advise, wherever possible, to should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month.

Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.

Read More

Question
Answer

Thank you for your questions and for your patience while I have been in touch with the biochemical scientists in our lab.

We haven't tested our biochemical products for biological activity in-house and haven't filtered Y-27632, however the lab scientists believe that this compound can be filtered through a 0.22 µM sterile filter. I looked throughthe method sections of several references and unfortunately couldn't find a definitive example of this being done.

I am sorry that I don't have further information regarding this compound. Please let me know if you have any further questions or if there is anything else that we can do for you.

Read More

Answer

Thank you for contacting us with your enquiry, and for your patience while I have been in touch with the lab regarding this question.

According to my contact in the lab, ourbiochemical compoundsare not made in a sterile environment, and as such we would recommend that sterilisation measures are taken prior to use, if a sterile solution is needed.

I hope this information will be useful, but please let me know if you have any further questions or if there is anything else that we can do for you, and I'll be happy to help. Have a great day!

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

登録