Anti-XRCC2 抗体 [EPR5149] - BSA and Azide free (ab248049)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5149] to XRCC2 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-XRCC2 antibody [EPR5149] - BSA and Azide free
XRCC2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5149] to XRCC2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248049 is the carrier-free version of ab124900.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5149 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248049の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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|
WB |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa).
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 32 kDa). |
ターゲット情報
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機能
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. -
配列類似性
Belongs to the RecA family. RAD51 subfamily. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm, Cytoskeleton, Nucleus - Information by UniProt
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参照データベース
- Entrez Gene: 7516 Human
- Entrez Gene: 57434 Mouse
- Entrez Gene: 499966 Rat
- Omim: 600375 Human
- SwissProt: O43543 Human
- SwissProt: Q9CX47 Mouse
- Unigene: 647093 Human
- Unigene: 143767 Mouse
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別名
- DKFZp781P0919 antibody
- DNA repair protein XRCC2 antibody
- X ray repair complementing defective repair in Chinese hamster cells 2 antibody
see all
画像
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All lanes : Anti-XRCC2 antibody [EPR5149] (ab124900) at 1/10000 dilution
Lane 1 : Human testis lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : SH-SY5Y lysate
Lane 5 : Caco 2 lysate
Lane 6 : Human kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDaThis data was developed using ab124900, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab248049 は 1 報の論文で使用されています。
- Kusi M et al. 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity. Cell Rep 38:110220 (2022). PubMed: 35021081