Key features and details
- Rabbit polyclonal to XRCC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
XRCC1 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to XRCC1
特異性This antibody recognises human XRCC1 (x-ray cross complementing factor -1), a base excision repair protein.
アプリケーション適用あり: WBmore details
交差が予測される動物種: Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide (Human) conjugated to KLH - which represented aportion within the last 100 amino acids of the human X-ray Repair Cross Complementing Protein 1 (GenBank PID 11425942).
特記事項Recently XRCC-1 has been shown to be physically associated with other DNA repair enzymes, including PARP, DNA ligase III and DNA polymerase beta.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Purine and pyrimidine synthesis
Our Abpromise guarantee covers the use of ab9147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 90 kDa (predicted molecular weight: 70 kDa).|
機能Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
配列類似性Contains 2 BRCT domains.
翻訳後修飾Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
細胞内局在Nucleus. Accumulates at sites of DNA damage.
- Information by UniProt
- DNA repair protein XRCC1 antibody
- RCC antibody
- X ray repair complementing defective repair in chinese hamster antibody
All lanes : Anti-XRCC1 antibody (ab9147) at 1 µg/ml
Lane 1 : Whole cell extract (50mcg) from mock treated HeLa cells.
Lane 2 : Whole cell extract (50mcg) from siRNA treated HeLa cells.
Predicted band size: 70 kDa
Observed band size: 76,90 kDa why is the actual band size different from the predicted?
ab9147 は 13 報の論文で使用されています。
- Zhang Z et al. Triptolide interferes with XRCC1/PARP1-mediated DNA repair and confers sensitization of triple-negative breast cancer cells to cisplatin. Biomed Pharmacother 109:1541-1546 (2019). PubMed: 30551406
- Zhang LH et al. Up-regulated Wnt1-inducible signaling pathway protein 1 correlates with poor prognosis and drug resistance by reducing DNA repair in gastric cancer. World J Gastroenterol 25:5814-5825 (2019). PubMed: 31636474
- Sun C et al. Mobile phone signal exposure triggers a hormesis-like effect in Atm(+/+) and Atm(-/-) mouse embryonic fibroblasts. Sci Rep 6:37423 (2016). WB ; Mouse . PubMed: 27857169
- Yousif AS et al. Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase. Proc Natl Acad Sci U S A 111:E1016-24 (2014). PubMed: 24591630
- Slupianek A et al. BCR/ABL stimulates WRN to promote survival and genomic instability. Cancer Res 71:842-51 (2011). WB ; Mouse . PubMed: 21123451