Anti-XPG 抗体 (ab224815)
Key features and details
- Rabbit polyclonal to XPG
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-XPG antibody
XPG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XPG -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human XPG aa 650-700. The exact sequence is proprietary. P28715
Database link: NP_000114.2 -
ポジティブ・コントロール
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab224815の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
WB |
1/2000 - 1/10000. Predicted molecular weight: 133 kDa.
|
特記事項 |
---|
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 133 kDa. |
ターゲット情報
-
機能
Single-stranded structure-specific DNA endonuclease involved in DNA excision repair. Makes the 3'incision in DNA nucleotide excision repair (NER). Acts as a cofactor for a DNA glycosylase that removes oxidized pyrimidines from DNA. May also be involved in transcription-coupled repair of this kind of damage, in transcription by RNA polymerase II, and perhaps in other processes too. -
関連疾患
Defects in ERCC5 are the cause of xeroderma pigmentosum complementation group G (XP-G) [MIM:278780]; also known as xeroderma pigmentosum VII (XP7). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-G patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities. -
配列類似性
Belongs to the XPG/RAD2 endonuclease family. XPG subfamily. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 2073 Human
- Omim: 133530 Human
- SwissProt: P28715 Human
- Unigene: 258429 Human
-
別名
- COFS 3 antibody
- COFS3 antibody
- DNA excision repair protein ERCC 5 antibody
see all
画像
-
All lanes : Anti-XPG antibody (ab224815) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Exposure time: 3 minutes -
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for XPG using ab224815 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining.
-
XPG was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224815 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab224815 at 1 µg/ml.
Lane 1: ab224815 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab224815 は 1 報の論文で使用されています。
- Edwards DS et al. BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation. Cell Rep 32:108166 (2020). PubMed: 32966794