製品の概要

  • 製品名
    Anti-XPA antibody [5A2]
    XPA 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [5A2] to XPA
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    His-tagged recombinant human XPA (Xeroderma Pigmentosum group A) protein

  • 特記事項
    Works on pure and partially purified protein but not on crude cell extract.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    5A2
  • ミエローマ
    Sp2
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab44 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 31 kDa.

ターゲット情報

  • 機能
    Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.
  • 組織特異性
    Expressed in various cell lines and in skin fibroblasts.
  • 関連疾患
    Defects in XPA are a cause of xeroderma pigmentosum complementation group A (XP-A) [MIM:278700]; also known as xeroderma pigmentosum type 1 (XP1). XP-A is a rare human autosomal recessive disease characterized by solar sensitivity, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Group A patients show the most severe skin symptoms and progressive neurological disorders.
  • 配列類似性
    Belongs to the XPA family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated by HERC2 leading to degradation by the proteasome.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA repair protein complementing XP A cells antibody
    • DNA repair protein complementing XP-A cells antibody
    • DNA repair protein complementing XPA cells antibody
    • Excision repair controlling antibody
    • Xeroderma pigmentosum 1 antibody
    • Xeroderma pigmentosum complementation group A antibody
    • Xeroderma pigmentosum group A complementing protein antibody
    • Xeroderma pigmentosum group A-complementing protein antibody
    • XP 1 antibody
    • XP1 antibody
    • xpa antibody
    • XPA_HUMAN antibody
    • Xpac antibody
    see all

プロトコール

参考文献

This product has been referenced in:
  • Wei Q  et al. Expression of nucleotide excision repair proteins in lymphocytes as a marker of susceptibility to squamous cell carcinomas of the head and neck. Cancer Epidemiol Biomarkers Prev 14:1961-6 (2005). Read more (PubMed: 16103444) »
See 1 Publication for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Answer

Thank you for your enquiry regarding ab44. We have not mapped the epitope recognized by this antibody therefore we do not know if it will or will not recognized your truncated protein. All our other antibodies against XPA are likely to recognize the non truncated form (e.g. polyclonal antibodies) as well as the truncated form or we do not know where the epitope is. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Answer

Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet, and at this time, we are unaware of any publications/references that feature the use of ab44. If you have any more questions, please contact us again.

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Answer

Thank you for getting back to us and sharing your recent results. We are very sorry to hear that you are still having problem with this antibody. The protocol we have sent it to you is a general ELISA method. Looking at our order record, we can inform you that we have sold several vials of this product without any problem. We get this product from an academic source and originally it has not been tested for ELISA. However, one of our customers reported in 2001 that it worked nicely on ELISA application. We published his comment on-line, please take a look at John Butler's review on the top panel of the datasheet. We would advise perhaps to contact this researcher (you can send an-email to him directly by clicking on his name) to get some more details of his protocol and discuss this issue further. Certainly, we can offer you a replacement vial - free of charge, it is possible that the vial you received may have gone off during shipping and/or storage. If you would like to get it, please do let us know. We look forward to hearing from you soon.

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Answer

Thank you for your enquiry. We are very sorry to hear that you are having problem with this product. I have searched our database and found that this is a popular selling product and your feedback is the first we have received about it not working. Therefore, at this stage, I would suggest that there is either a problem with the vial you received, or modifications to your protocol are needed to obtain a positive result. I would like to make the following suggestions: It is very important to emphasize that when you coat the wells with the antigen, always use coating buffer instead of water. For ELISA, we would suggest using 1% (v/w) BSA and the blocking should be at least for 60 min. 10 min is too short!!! I will send you detailed protocol for you in my next e-mail.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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