Anti-XIAP 抗体 (ab2541)
Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-XIAP antibody
XIAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XIAP -
由来種
Rabbit -
特異性
This antibody detects the 53 kDa human XIAP protein. It does not cross-react with other apoptosis inhibitors. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Available on request. (Peptide available as ab38275.)
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 30% Glycerol, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab2541 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). | |
| ICC/IF | 1/200. |
ターゲット情報
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機能
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. -
組織特異性
Ubiquitous, except peripheral blood leukocytes. -
関連疾患
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
配列類似性
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
ドメイン
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. -
翻訳後修飾
Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 331 Human
- Entrez Gene: 11798 Mouse
- Entrez Gene: 63879 Rat
- Omim: 300079 Human
- SwissProt: P98170 Human
- SwissProt: Q60989 Mouse
- SwissProt: Q9R0I6 Rat
- Unigene: 356076 Human
see all -
別名
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)This image is courtesy of an Abreview submitted by Dr Kirk McManusab2541 (1/200) staining XIAP in HeLa cells (green). Cells were permeabilised with 0.5% Triton-X100/ PBS, fixed in methanol (-20oC, 10min) and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to abreview for further experimental details. -
Western blot - Anti-XIAP antibody (ab2541)All lanes : Anti-XIAP antibody (ab2541) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 12 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
参考文献
ab2541 は 7 報の論文で使用されています。
- Dong X et al. Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-ß1/Smad Signaling Pathway in Rats with Rheumatoid Arthritis. Evid Based Complement Alternat Med 2019:8614034 (2019). PubMed: 31929822
- Li Y et al. Dual targeting of survivin and X-linked inhibitor of apoptosis protein suppresses the growth and promotes the apoptosis of gastric cancer HGC-27 cells. Oncol Lett 16:3489-3498 (2018). PubMed: 30127953
- Bao J et al. Fmr1 protects cardiomyocytes against lipopolysaccharide-induced myocardial injury. Exp Ther Med 16:1825-1833 (2018). PubMed: 30186407
- Zhang QY et al. FAM46C is critical for the anti-proliferation and pro-apoptotic effects of norcantharidin in hepatocellular carcinoma cells. Sci Rep 7:396 (2017). WB ; Human . PubMed: 28341836
- Hsu KH et al. Anti-apoptotic effects of osteopontin through the up-regulation of Mcl-1 in gastrointestinal stromal tumors. World J Surg Oncol 12:189 (2014). WB ; Human . PubMed: 24947165
- Lopergolo A et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB ; Human . PubMed: 19223905
- Shrikhande SV et al. Silencing of X-linked inhibitor of apoptosis (XIAP) decreases gemcitabine resistance of pancreatic cancer cells. Anticancer Res 26:3265-73 (2006). IHC (PFA fixed) . PubMed: 17094439
