Anti-XIAP 抗体 (ab2541)

Reviews (1)Q&A (5)References (7)

Key features and details

  • Rabbit polyclonal to XIAP
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-XIAP antibody
    XIAP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to XIAP

  • 由来種

    Rabbit

  • 特異性

    This antibody detects the 53 kDa human XIAP protein. It does not cross-react with other apoptosis inhibitors.

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Available on request. (Peptide available as ab38275.)

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
ICC/IF 1/200.

ターゲット情報

  • 機能

    Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.

  • 組織特異性

    Ubiquitous, except peripheral blood leukocytes.

  • 関連疾患

    Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

  • 配列類似性

    Belongs to the IAP family.
    Contains 3 BIR repeats.
    Contains 1 RING-type zinc finger.

  • ドメイン

    The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.

  • 翻訳後修飾

    Ubiquitinated and degraded by the proteasome in apoptotic cells.
    Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AP 13 antibody
    • API3 antibody
    • Apoptosis Inhibitor 3 antibody
    • Baculoviral IAP repeat containing 4 antibody
    • Baculoviral IAP Repeat Containing Protein 4 antibody
    • Baculoviral IAP repeat-containing protein 4 antibody
    • BIRC 4 antibody
    • BIRC4 antibody
    • E3 ubiquitin-protein ligase XIAP antibody
    • hIAP-3 antibody
    • hIAP3 antibody
    • HILP antibody
    • IAP 3 antibody
    • IAP like protein antibody
    • IAP-3 antibody
    • IAP-like protein antibody
    • IAP3 antibody
    • ILP 1 antibody
    • ILP antibody
    • ILP1 antibody
    • Inhibitor of apoptosis protein 3 antibody
    • Inhibitor of Apoptosis X Linked antibody
    • Mammalian IAP Homologue A antibody
    • MIHA antibody
    • X linked IAP antibody
    • X linked inhibitor of apoptosis antibody
    • X linked inhibitor of apoptosis E3 ubiquitin protein ligase antibody
    • X linked inhibitor of apoptosis protein antibody
    • X-linked IAP antibody
    • x-linked inhibitor of apopotosis proteins antibody
    • X-linked inhibitor of apoptosis protein antibody
    • Xiap antibody
    • XIAP_HUMAN antibody
    • XLP2 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)
    Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)This image is courtesy of an Abreview submitted by Dr Kirk McManus
    ab2541 (1/200) staining XIAP in HeLa cells (green). Cells were permeabilised with 0.5% Triton-X100/ PBS, fixed in methanol (-20oC, 10min) and counterstained with DAPI (red) in order to highlight the nucleus. Please refer to abreview for further experimental details.

    See Abreview

  • Western blot - Anti-XIAP antibody (ab2541)
    Western blot - Anti-XIAP antibody (ab2541)
    All lanes : Anti-XIAP antibody (ab2541) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 12 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)
    Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab2541)
    ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
    The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

参考文献 (7)

ab2541 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2541 は 7 報の論文で使用されています。

  • Dong X  et al. Effect of Jiawei Fengshining on Synovial Cell Apoptosis and TGF-ß1/Smad Signaling Pathway in Rats with Rheumatoid Arthritis. Evid Based Complement Alternat Med 2019:8614034 (2019). PubMed: 31929822
  • Li Y  et al. Dual targeting of survivin and X-linked inhibitor of apoptosis protein suppresses the growth and promotes the apoptosis of gastric cancer HGC-27 cells. Oncol Lett 16:3489-3498 (2018). PubMed: 30127953
  • Bao J  et al. Fmr1 protects cardiomyocytes against lipopolysaccharide-induced myocardial injury. Exp Ther Med 16:1825-1833 (2018). PubMed: 30186407
  • Zhang QY  et al. FAM46C is critical for the anti-proliferation and pro-apoptotic effects of norcantharidin in hepatocellular carcinoma cells. Sci Rep 7:396 (2017). WB ; Human . PubMed: 28341836
  • Hsu KH  et al. Anti-apoptotic effects of osteopontin through the up-regulation of Mcl-1 in gastrointestinal stromal tumors. World J Surg Oncol 12:189 (2014). WB ; Human . PubMed: 24947165
  • Lopergolo A  et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. Br J Cancer 100:739-46 (2009). WB ; Human . PubMed: 19223905
  • Shrikhande SV  et al. Silencing of X-linked inhibitor of apoptosis (XIAP) decreases gemcitabine resistance of pancreatic cancer cells. Anticancer Res 26:3265-73 (2006). IHC (PFA fixed) . PubMed: 17094439

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1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-XIAP antibody

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0.5% Triton-X100 in PBS
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Dr. Kirk Mcmanus

Verified customer

投稿 Nov 30 2007

Question

We have a customer trying to perform western blotting with the XIAP polyclonal antibody (Catalog Number ab2541). They could not obtain a specific band on the blot. They tried few protocol with room temperature incubation as well as overnight incubation with similar results. Blotting with other anitbody suggested the lysates are fine. Initially, what is your recommendation for primary antibody incubation condition?

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Answer

Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. Initially I would like to recommend that they apply the antibody at a dilution of 1:100 at 4oC overnight using 3% BSA/TBST as a blocking agent to determine whether the antibody is to blame. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

BATCH NUMBER 95586 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining (once) High background (twice) Weak signal even in negative control (twice) SAMPLE paraffin embedded human tissues PRIMARY ANTIBODY XIAP (AbCam); overnight incubation at 4?C; dilution 1:100, 1:200, longer washings with TBS-BSA 0.1% SECONDARY ANTIBODY Goat anti-rabbit (KPL); 45 minutes incubation, three times wash with TBS-BSA 0.1% DETECTION METHOD Streptavidine peroxidase followed by TBS-BSA 0.1% washing 3 times and then DAB-chromogen method for detection POSITIVE AND NEGATIVE CONTROLS USED Rabbit IgG used as negative control To the best of my knowledge there is no positive control tissue for IHC. ANTIBODY STORAGE CONDITIONS -20?C, aliquoted FIXATION OF SAMPLE Paraformaldehyde ANTIGEN RETRIEVAL Microwave boiling for 10 minutes in citrate buffer PERMEABILIZATION STEP Not used BLOCKING CONDITIONS Normal Goat serum (45 minutes) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? 1. Dakocytomation Antibody diluent with background reducing components 2. Washing with Tween to reduce background ADDITIONAL NOTES Can you supply a specific negative control for this XIAP antibody which I could try in IHC on paraffin embedded tissues?

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Answer

We have been informed by the source of ab2541 that western blots showed mouse intestine was positive for XIAP. We do not have a negative tissue sample available unfortunately. I hope this information will be useful, please do not hesitate to contact us if we can be of further assistance,

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Question

BATCH NUMBER 95586 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No staining (once) High background (twice) Weak signal even in negative control (twice) SAMPLE paraffin embedded human tissues PRIMARY ANTIBODY XIAP (AbCam); overnight incubation at 4?C; dilution 1:100, 1:200, longer washings with TBS-BSA 0.1% SECONDARY ANTIBODY Goat anti-rabbit (KPL); 45 minutes incubation, three times wash with TBS-BSA 0.1% DETECTION METHOD Streptavidine peroxidase followed by TBS-BSA 0.1% washing 3 times and then DAB-chromogen method for detection POSITIVE AND NEGATIVE CONTROLS USED Rabbit IgG used as negative control To the best of my knowledge there is no positive control tissue for IHC. ANTIBODY STORAGE CONDITIONS -20?C, aliquoted FIXATION OF SAMPLE Paraformaldehyde ANTIGEN RETRIEVAL Microwave boiling for 10 minutes in citrate buffer PERMEABILIZATION STEP Not used BLOCKING CONDITIONS Normal Goat serum (45 minutes) HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 6 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? No WHAT STEPS HAVE YOU ALTERED? 1. Dakocytomation Antibody diluent with background reducing components 2. Washing with Tween to reduce background ADDITIONAL NOTES Can you supply a specific negative control for this XIAP antibody which I could try in IHC on paraffin embedded tissues?

Read More
Answer

I'm sorry to hear you are having problems with ab2541 in IHC. As we have not tested the antibody in this application it is very difficult to find out what the problem might be and I am waiting to hear back from the source of the antibody regarding positive and negative controls to help you. I would like to suggest to consider different antigen retrieval methods (e.g. enzymatic) and adding triton x100 (0.3%v/v) in the buffer used to dilute both primary and secondary antibodies to maximise permeabilization and penetration of the antibodies. I will forward to you the information from the source of the antibody as soon as I receive it, my apologies for the delay, Thank you for your patience,

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Question

can you tell me if this antibody cross reacts with rat and will it IP?

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Answer

This antibody has not been tested on rat or IP so we have no further information on this.

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Question

please can you send me the antigen peptide sequence that has been use to raise this antibody thank you

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Answer

The immunogen is a 12 a.a. peptide around residue 240 of human XIAP. However, we are unable to disclose the detail sequence, as it is proprietary.

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