Anti-XIAP 抗体 (ab21278)
Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-XIAP antibody
XIAP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XIAP -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to 13 amino acids at the C-terminus of human XIAP.
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ポジティブ･コントロール
- Human kidney cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
特記事項（精製）
Purified by ion exchange chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab21278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 2 µg/ml.
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| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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| WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa.
Validated in human samples only. |
| 特記事項 |
|---|
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IHC-P
Use a concentration of 2 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 55 kDa. Validated in human samples only. |
ターゲット情報
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機能
Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. -
組織特異性
Ubiquitous, except peripheral blood leukocytes. -
関連疾患
Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma. -
配列類似性
Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger. -
ドメイン
The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains. -
翻訳後修飾
Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 331 Human
- Entrez Gene: 11798 Mouse
- Omim: 300079 Human
- SwissProt: P98170 Human
- SwissProt: Q60989 Mouse
- Unigene: 356076 Human
- Unigene: 259879 Mouse
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別名
- AP 13 antibody
- API3 antibody
- Apoptosis Inhibitor 3 antibody
see all
画像
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Western blot - Anti-XIAP antibody (ab21278)Lane 1 : Anti-XIAP antibody (ab21278) at 0.5 µg/ml
Lane 2 : Anti-XIAP antibody (ab21278) at 1 µg/ml
Lane 3 : Anti-XIAP antibody (ab21278) at 2 µg/ml
All lanes : Human kidney lysate
Predicted band size: 55 kDaWestern blot analysis of XIAP in human kidney cell lysate using ab21278 at a concentration of 0.5
µ g/ml (lane A), 1µ g/ml (lane B) and 2µ g/ml (lane C). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XIAP antibody (ab21278)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse kidney tissue staining XIAP with ab21278 at 20µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XIAP antibody (ab21278)Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse kidney tissue labeling XIAP with Anti-XIAP antibody (ab21278) at 5μg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XIAP antibody (ab21278)ab21278 at 2µg/ml staining XIAP in human kidney tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab21278)ICC/IF image of ab21278 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21278, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-XIAP antibody (ab21278)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (39)
ab21278 は 39 報の論文で使用されています。
- Yu B et al. Circ_0000527 Promotes Retinoblastoma Progression through Modulating miR-98-5p/XIAP Pathway. Curr Eye Res 46:1414-1423 (2021). PubMed: 33629639
- Sun F et al. LncRNA KCNQ1OT1 attenuates sepsis-induced myocardial injury via regulating miR-192-5p/XIAP axis. Exp Biol Med (Maywood) 245:620-630 (2020). PubMed: 32102564
- Zhang H et al. Hsa_circ_0067997 promotes the progression of gastric cancer by inhibition of miR-515-5p and activation of X chromosome-linked inhibitor of apoptosis (XIAP). Artif Cells Nanomed Biotechnol 47:308-318 (2019). PubMed: 30688097
- Chen JC et al. Enhanced cytotoxicity of human hepatocellular carcinoma cells following pretreatment with sorafenib combined with trichostatin A. Oncol Lett 17:638-645 (2019). PubMed: 30655811
- Baranov SV et al. Mitochondria modulate programmed neuritic retraction. Proc Natl Acad Sci U S A 116:650-659 (2019). PubMed: 30584104