Anti-XAF1 抗体 (ab17204)
Key features and details
- Rabbit polyclonal to XAF1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-XAF1 antibody
XAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XAF1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide (Human) (within the last 35 amino acids of C terminal end).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17204の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 18485100
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WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band.
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. PubMed: 18485100 |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa. A higher 50kDa band is present at times (lot dependent). This band is not blocked by the immunogen. We do not know the identity of this extra band. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. -
組織特異性
Widely expressed. Expression is frequently down-regulated in cancer cell lines. Isoform 5 is widely expressed. Expressed in placenta (at protein level). -
配列類似性
Contains 1 TRAF-type zinc finger. -
発生段階
Selectively expressed in third trimester placenta. -
細胞内局在
Nucleus and Cytoplasm. Nucleus. Mitochondrion. Found in the cytoplasm and nucleus of placental syncytiotrophoblasts. Translocates to mitochondria upon TNF-alpha treatment. - Information by UniProt
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参照データベース
- Entrez Gene: 54739 Human
- Entrez Gene: 327959 Mouse
- Entrez Gene: 679600 Rat
- Omim: 606717 Human
- SwissProt: Q6GPH4 Human
- SwissProt: Q5NBU8 Mouse
- Unigene: 441975 Human
- Unigene: 291131 Mouse
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別名
- BIRC 4 binding protein antibody
- BIRC4 binding protein antibody
- BIRC4-binding protein antibody
see all
画像
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Lane 1 : Anti-XAF1 antibody (ab17204) at 0.5 µg/ml
Lane 2 : Anti-XAF1 antibody (ab17204) at 1 µg/ml
Lane 3 : Anti-XAF1 antibody (ab17204) at 2 µg/ml
Lane 1 : Human spleen lysate
Lanes 2-3 : Human spleen lysate
Observed band size: 32 kDa why is the actual band size different from the predicted?Western blot analysis of XAF1 in human spleen lysate with ab17204 at
Lane A: 0.5 ug/ml
Lane B: 1ug/ml
Lane C: 2ug/ml
Higher 50kDa band presence is lot dependent. This band is not blocked by the immunogen. -
ab17204 at 2µg/ml staining XAF1 in human spleen tissue by IHC
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab17204 は 13 報の論文で使用されています。
- Sun G et al. WZY-321 triggers glioma cell apoptosis via XAF1 up-regulation caused by MTM-mediated miR-873 down-regulation. J Cancer 13:2312-2321 (2022). PubMed: 35517406
- Zhu C et al. SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. J Exp Med 216:743-756 (2019). PubMed: 30842236
- Xu T et al. Gene Amplification-Driven Long Noncoding RNA SNHG17 Regulates Cell Proliferation and Migration in Human Non-Small-Cell Lung Cancer. Mol Ther Nucleic Acids 17:405-413 (2019). PubMed: 31310946
- Jiang X et al. Bisphenol A induced male germ cell apoptosis via IFNß-XAF1-XIAP pathway in adult mice. Toxicol Appl Pharmacol 355:247-256 (2018). PubMed: 30017639
- Jeong SI et al. XAF1 forms a positive feedback loop with IRF-1 to drive apoptotic stress response and suppress tumorigenesis. Cell Death Dis 9:806 (2018). PubMed: 30042418