Application
Western blot
Sample
Human Cell lysate - whole cell (head and neck cancer)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
20 µg
Treatment
drug 48h
Specification
head and neck cancer
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit igG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 55 kDa Non-specific: 50 kDa
Additional data
Additional Notes
too much non specific band
but band is very clear
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 21 2015