Mouse IGFBP5 ELISA Kit (ab208345)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 73 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse IGFBP5 ELISA Kit
IGFBP5 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Mouse serum 5 3.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Mouse serum 3 2.4% -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
73 pg/ml -
検出範囲
125 pg/ml - 8000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 104 102% - 106% Serum 88 83% - 96% Hep Plasma 93 80% - 104% Cit plasma 98 92% - 102% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse
非交差種: Goat, Cow, Pig -
製品の概要
Mouse IGFBP5 ELISA Kit (ab208345) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP5 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP5 with 73 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Insulin-like growth factor-binding protein 5 (IGFBP5) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP5 can also alter the interaction of IGFs with their receptors.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10X Mouse IGFBP5 Capture Antibody 1 x 600µl 10X Mouse IGFBP5 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR2 1 x 6ml Mouse IGFBP5 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
組織特異性
Osteosarcoma, and at lower levels in liver, kidney and brain. -
配列類似性
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
細胞内局在
Secreted. - Information by UniProt
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別名
- IBP 5
- IBP-5
- IBP5
see all -
参照データベース
- Entrez Gene: 16011 Mouse
- SwissProt: Q07079 Mouse
- Unigene: 405761 Mouse
関連製品
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Alternative Versions
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ELISA kits
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Related Products
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of IGFBP5 were measured in duplicates, interpolated from the IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:600, plasma 1:800, and plasma (heparin) 1:800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2,058 ng/mL in serum, 3,670 ng/mL in plasma (citrate), and 3,865 ng/mL in plasma (heparin).
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The concentrations of IGFBP5 in rat serum were measured in duplicates, interpolated from the mouse IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are rat serum 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2691 ng/mL in rat serum.
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab208345 は 1 報の論文で使用されています。
- Huang X et al. Molecular characterization of a precision-cut rat liver slice model for the evaluation of antifibrotic compounds. Am J Physiol Gastrointest Liver Physiol 316:G15-G24 (2019). PubMed: 30406699