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Anti-Wnt7a 抗体 (ab100792)

Reviews (2)Q&A (3)References (19)
Western blot - Anti-Wnt7a antibody (ab100792)
  • Western blot - Anti-Wnt7a antibody (ab100792)
  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (ab100792)

Key features and details

  • Rabbit polyclonal to Wnt7a
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Wnt7a antibody [EPR23471-125] (ab274321)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Wnt7a antibody
    Wnt7a 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Wnt7a

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Chicken, Chimpanzee, Gorilla, Orangutan

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Recombinant human Wnt7a protein (ab116171) can be used as positive control in WB. In Western Blot, ab100792 gave a positive signal in Human kidney tissue lysate, human and mouse kidney lysate, HEPg2 cells, COLO 205 whole cell lysate and human colon tumor tissue. This antibody gave a positive result in ICC in HepG2 cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab100792の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).

Abcam recommends using 5% BSA as the blocking agent.
ICC/IF (1)
Use a concentration of 5 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 39 kDa).

Abcam recommends using 5% BSA as the blocking agent.
ICC/IF
Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. Signaling by Wnt-7a allows sexually dimorphic development of the mullerian ducts.

  • 組織特異性

    Expression is restricted to placenta, kidney, testis, uterus, fetal lung, and fetal and adult brain.

  • 関連疾患

    Defects in WNT7A are the cause of limb/pelvis-hypoplasia/aplasia syndrome (LPHAS) [MIM:276820]; also known as absence of ulna and fibula with severe limb deficiency. LPHAS is a limb-malformation disorder characterized by various degrees of limb aplasia/hypoplasia and joint dysplasia.
    Defects in WNT7A are a cause of Fuhrmann syndrome (FUHRS) [MIM:228930]; also known as fibular aplasia or hypoplasia femoral bowing and poly- syn- and oligodactyly. Fuhrmann syndrome is a distinct limb-malformation disorder characterized also by various degrees of limb aplasia/hypoplasia and joint dysplasia.

  • 配列類似性

    Belongs to the Wnt family.

  • 細胞内局在

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Protein Wnt-7a antibody
    • Protein Wnt-7a precursor antibody
    • Proto oncogene Wnt7a protein antibody
    • proto-oncogene wnt7a protein antibody
    • wingless-type MMTV integration site family, member 7A antibody
    • Wnt family member 7A antibody
    • WNT7A antibody
    • WNT7A_HUMAN antibody
    see all

画像

  • Western blot - Anti-Wnt7a antibody (ab100792)
    Western blot - Anti-Wnt7a antibody (ab100792)
    All lanes : Anti-Wnt7a antibody (ab100792) at 1 µg/ml

    Lane 1 : Human Kidney lysate
    Lane 2 : Mouse Kidney lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : COLO 205 whole cell lysate
    Lane 5 : Human Colon tumour tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Predicted band size: 39 kDa



    Exposure: 4 minutes

    Blocking buffer: 2% BSA

    Gel type: MOPS

    Observed band size: 41 kDa

    Additional bands: 50 kDa, 27kDa

  • Western blot - Anti-Wnt7a antibody (ab100792)
    Western blot - Anti-Wnt7a antibody (ab100792)
    Anti-Wnt7a antibody (ab100792) at 1 µg/ml + Human kidney tissue lysate - total protein (ab30203) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39 kDa
    Observed band size: 41 kDa why is the actual band size different from the predicted?
    Additional bands at: 15 kDa, 28 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (ab100792)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (ab100792)
    ICC/IF image of ab100792 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab100792, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 5µg/ml.

参考文献 (19)

ab100792 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab100792 は 19 報の論文で使用されています。

  • Yang L  et al. Wnt7a promotes the osteogenic differentiation of human mesenchymal stem cells. Int J Mol Med 47:N/A (2021). PubMed: 33846764
  • Zhang R  et al. lncRNA BCAR4 sponges miR-370-3p to promote bladder cancer progression via Wnt signaling. Int J Mol Med 45:578-588 (2020). PubMed: 31894304
  • Taxier LR  et al. Dickkopf-1 blocks 17ß-estradiol-enhanced object memory consolidation in ovariectomized female mice. Horm Behav 114:104545 (2019). PubMed: 31228421
  • Wang L  et al. miR-127 suppresses gastric cancer cell migration and invasion via targeting Wnt7a. Oncol Lett 17:3219-3226 (2019). PubMed: 30867752
  • Vallon M  et al. A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability. Cell Rep 25:339-349.e9 (2018). PubMed: 30304675
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Wnt7a antibody

 Poor
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain)
Permeabilization
Yes - 0.5% with Triton X 100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Gulnaz Begum

Verified customer

投稿 Oct 31 2017

Western blot abreview for Anti-Wnt7a antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (C2C12)
Gel Running Conditions
Reduced Denaturing (10% GEL)
Loading amount
30 µg
Specification
C2C12
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 15 2016

Question

Dear
Thanks for your kindly reply, after contacting with this customer and passed these suggestions to him, then he modified his experiment step as your suggestion, but it didn’t improve his results, he also indicated he didn’t has any positive control can test in this experiment, and he said the band which at 48kDa will disappear after he reused this antibody, he offered the RT-PCR data to prove this protein will expression in this sample, please check it and help this customer to solve the problem.
Thanks for your kindly help

Best regards

Read More
Answer

Thank you for your reply.

I contacted with my lab colleagues who confirmed this lot has successfully passed our QC controls, and as with previous batches, it should be detecting a band at ˜40KDa, rather than 48KDa.

Please find attached one of the last blots obtained comparing our new batch (lane 1) with the batch you have tested (lane 3).

As you have mentioned, the band at ˜48KDa disappear when retesting the antibody, it may well be a non specific band. However, attending to the image sent on your first email, there is a weak band showing at ˜43 kDa that could be reduced by the milk blocking. I would therefore recommend you to use the same blocking agent used in our lab: 5%BSA, in an attempt to increase the target’s band intensity.

In any case, all of our products are covered by our Abpromise guarantee; which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 4 months of purchase.

To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

I hope these tips help, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again and I’ll be more than happy to help you further.

Read More

Question

Dear technical support team:
This customer has purchased ab100792 (Anti-Wnt7a antibody) and has conducted the wb several times with human sample. The results show two bands, therefore, he would like to ask for your help to determine which the major band is, and could you please offer any suggestion to help him?
I also attached his image in this letter and his experiment step as follow:
1. Order details:
Abcam product code: ab100792
Batch number: XXXXXX
Po: XXXXXXX
Antibody storage conditions (temperature/reconstitution etc) -20°C
2. Please describe the problem (high background, wrong band size, more bands, no band etc). Two bands
3. On what material are you testing the antibody in WB?
· Species:
· What’s cell line or tissue: Lung cancer cell line
· Cell extract or Nuclear extract: whole cell lysate
· Purified protein or Recombinant protein:
3. The lysate
How much protein was loaded: 40ug
What lysis buffer was used:
What protease inhibitors were used: protein inhibitor set III, Roche inhibitor
What loading buffer was used: 2X Laemmli buffer
Phosphatase inhibitors
· Did you heat the samples: temperature and time: 100℃ 5min
4. Electrophoresis/Gel conditions/ Transfer conditions
Reducing or non reducing gel:
Reducing agent:
Gel percentage :
Transfer conditions: (Type of membrane, Protein transfer verified):
5. Blocking conditions
Buffer: TBS
Blocking agent: milk, BSA, serum, what percentage: 3% milk
Incubation time: 2h
Incubation temperature: RT
6. Primary Antibody
Species:
Reacts against:
· At what dilution(s) have you tested this antibody: 1:1500
· What dilution buffer was used: 3% milk in TBS
· Incubation time: overnight
· Incubation temperature: 4℃
· What washing steps were done: 3 times
7. Secondary Antibody
Species:
Reacts against:
At what dilution(s) have you tested this antibody: 1:3000
Incubation time: 2 h
Wash steps: Blocking buffer wash 3 time
Fluorochrome or enzyme conjugate:
Do you know whether the problems you are experiencing come from the secondary? no
8. Detection method
ECl, ECl+, other detection method:ECl
9. Did you apply positive and negative controls along with the samples? Please specify.
10. Optimization attempts
· How many times have you tried the Western? 5
· Have you eliminated the possibility that any background bands could be due to the secondary antibody? (Run a “No primary” control): no
· Do you obtain the same results every time e.g. are background bands always in the same place? yes
· What steps have you altered?
Could you please help this customer to solve the problem?Thanks for your kindly help

Read More
Answer

Thank you for taking the time to complete our questionnaire.

The predicted band size for this protein is 39KDa, however, attending at our in-house tests, the observed band size appears at approximately 41KDa, and other unknown additional bands are shown at lower molecular weights.

In order to identify the correct band for the protein I would suggest using a positive control. Human kidney tissue lysate has been proved to express this protein, and therefore could be used as such.

In an attempt to improve the results observed I would suggest increasing the percentage of milk to 5% as well as optimising the sample preparation lysing your tissue with RIPA buffer and adding phosphatase inhibitors.

I would also recommend decreasing the protein load to 20ug, and using less concentrate dilution of primary antibody (e.g. 1/1000).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

These are the translated AA seq's I'm working with let me know what you can find out. DVL1 Pig aa seq K G G A V A A D G R T E P G D Met L L Q V N D V N F E N Met S N D D A V R V L R E I V S Q T G P I S L T V A K C W D P S P R S Y F T I P R A D P V R P I D P A A W L S H T A A L T G A L P R Y G T S P C S S A V T R T S S S S L T S S V P G A P Q L E E A P L T V K S D Met G A V V R V Met Q L P D S G L E I R D R Met W L K I T I A N A V I G A D V V D W L Y T H L E G F R E R R E A R R Y A S S Met L K R G F L R H T V N K V T F S E Q C Y Y V F G D L C S N L A A L N L N S G S S G A S D Q D T L A P L P H P A A P W P L G Q G Y P Y Q Y P G P P P C F P P A Y Q D P G F S Y G S G S A G S Q Q S E G S K S S G S T R S A G G S S R R A L G R E K E S R S A G A G G S G S E S D H T A P S G A G G S G W R E R P A S Q L S R G S S P R S Q A S A A A P G L P P L H P L T K A Y S V V G G P P G G P P V R E L A A V P P E L T G S R Q S F Q K A Met G N P C E F F V D I Met wnt7a pig aa seq N L H N N E A G R K I L E E N Met K L E C K C H G V S G S C T T K T C W T T L P Q F R E L G Y V L K D K Y N E A V H V E P V R A S R N K R P T F L K I K K P L S Y R K P Met D T D L V Y I E K S P N Y C E E D P V T G S V G T Q G R A C N K T A P Q A S G C D L Met C C G R G Y N T H Q Y A R V W Q C N C K F H W C C Y V K C N T C S E R T E V Y T C K

Read More
Answer

Thank you for calling Abcam. I have done the alignment between the sequences you sent and the immunogen sequences for the antibodies we discussed. For DVL1, there is 75% match between the sequence you sent and the immunogen sequence used for ab106914. We normally look for a 85% similarity before we would recommended trying an antibody, so in this case I do not think the antibody will react with pig. For Wtn7a, there is a 100% match between your sequence and the immunogen sequence used for ab100792. Therefore I would be very confident that this antibody would recognize pig Wnt7a. Please let me know if there is anything else I can help you with.

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