Key features and details
- Rabbit polyclonal to Wnt3a
- Suitable for: WB, IHC-Fr, IP, IHC - Wholemount, ICC/IF
- Reacts with: Mouse, Chicken, Human, Zebrafish
- Isotype: IgG
Wnt3a 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Wnt3a
アプリケーション適用あり: WB, IHC-Fr, IP, IHC - Wholemount, ICC/IFmore details
種交差性交差種: Mouse, Chicken, Human, Zebrafish
交差が予測される動物種: Xenopus laevis, a wide range of other species
- In WB, this antibody gave a positive signal when tested against recombinant Wnt3a protein.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
精製度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab28472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).Can be blocked with Human Wnt3a peptide (ab23327).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21048974|
|IP||Use at an assay dependent concentration. PubMed: 21740904|
|IHC - Wholemount||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
機能Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.
組織特異性Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate.
配列類似性Belongs to the Wnt family.
細胞内局在Secreted > extracellular space > extracellular matrix.
- Information by UniProt
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
Anti-Wnt3a antibody (ab28472) at 1 µg/ml + Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 39 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.
ab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
ab28472 staining Wnt3a in the dentate gyrus of rat bain tissue by Immunohistochemistry (Frozen sections).
Brains were sectioned in the coronal plane at 20 µm. The slides were left in a fume hood to dry overnight, then stored at -70°C. For each rat twelve sections through the dorsal hippocampus were analyzed. For single staining with Wnt3a, sections were incubated with ab28472 at 5 ug/ml in 0.5% Triton with 1% goat serum) overnight in a humidified chamber at room temperature. The following day, after two washes with KPBS, they were incubated in Alexa Fluor 568 goat anti-rabbit (1/200 in 0.5% Triton) for one hour, and washed twice with KPBS and covered slipped.
IHC-Wholemount image of Wnt3a (ab28472) on zebrafish larvae. Whole zebrafish larvae were fixed in paraformaldehyde and then permeabilized in proteinase K, before blocking in 5% serum for 1 hour at RT. The image above is a ventral view of the head, showing the ceratohyal cartilage (of the jaw).
ab28472 は 81 報の論文で使用されています。
- Huang L et al. Long non-coding RNA NCK1-AS1 promotes the tumorigenesis of glioma through sponging microRNA-138-2-3p and activating the TRIM24/Wnt/ß-catenin axis. J Exp Clin Cancer Res 39:63 (2020). PubMed: 32293515
- Kim JH et al. Development of finasteride polymer microspheres for systemic application in androgenic alopecia. Int J Mol Med 43:2409-2419 (2019). PubMed: 30942390
- Bastías-Candia S et al. Wnt Signaling Upregulates Teneurin-3 Expression via Canonical and Non-canonical Wnt Pathway Crosstalk. Front Neurosci 13:505 (2019). PubMed: 31156379
- Shi Q et al. Ginsenoside Rg1 protects human retinal pigment epithelial ARPE-19 cells from toxicity of high glucose by up-regulation of miR-26a. Life Sci 221:152-158 (2019). PubMed: 30763577
- Song H et al. BRD4 promotes the stemness of gastric cancer cells via attenuating miR-216a-3p-mediated inhibition of Wnt/ß-catenin signaling. Eur J Pharmacol 852:189-197 (2019). PubMed: 30876979