Anti-Wnt3a 抗体 (ab19925)
Key features and details
- Rabbit polyclonal to Wnt3a
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Wnt3a antibody
Wnt3a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Wnt3a -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Chicken, Human, Xenopus laevis, Zebrafish, a wide range of other species
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免疫原
Synthetic peptide corresponding to Human Wnt3a aa 200-300 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23326) -
ポジティブ･コントロール
- In WB, this antibody gave a positive signal when tested against a partial recombinant protein. IHC-P: Mouse placenta FFPE tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab19925の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Can be blocked with Wnt3a peptide (ab23326).
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| IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). Can be blocked with Wnt3a peptide (ab23326). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. -
組織特異性
Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 395396 Chicken
- Entrez Gene: 89780 Human
- Entrez Gene: 22416 Mouse
- Entrez Gene: 378679 Xenopus laevis
- Omim: 606359 Human
- SwissProt: Q2LMP1 Chicken
- SwissProt: P56704 Human
- SwissProt: P27467 Mouse
see all -
別名
- Protein Wnt 3a Precursor antibody
- Protein Wnt-3a antibody
- Wingless type MMTV integration site family member 3A antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody (ab19925)
IHC image of Wnt3a staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19925, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
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Western blot - Anti-Wnt3a antibody (ab19925)Anti-Wnt3a antibody (ab19925) at 1 µg/ml + Recombinant mouse Wnt3a, AAs 19-352 with a 16 AA n-terminal non-Wnt3a sequence at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/15000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
ab19925 detects a band of the expected size for recombinant mouse Wnt3a.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (14)
ab19925 は 14 報の論文で使用されています。
- Vaivads M et al. Cleft Candidate Genes and Their Products in Human Unilateral Cleft Lip Tissue. Diseases 9:N/A (2021). PubMed: 33917041
- Moyle LA et al. Three-dimensional niche stiffness synergizes with Wnt7a to modulate the extent of satellite cell symmetric self-renewal divisions. Mol Biol Cell N/A:mbcE20010078 (2020). PubMed: 32491970
- Kerdidani D et al. Wnt1 silences chemokine genes in dendritic cells and induces adaptive immune resistance in lung adenocarcinoma. Nat Commun 10:1405 (2019). PubMed: 30926812
- Jiang Z et al. Inactivation of the Wnt/ß-catenin signaling pathway underlies inhibitory role of microRNA-129-5p in epithelial-mesenchymal transition and angiogenesis of prostate cancer by targeting ZIC2. Cancer Cell Int 19:271 (2019). PubMed: 31649488
- Li H et al. CLK3 Is A Direct Target Of miR-144 And Contributes To Aggressive Progression In Hepatocellular Carcinoma. Onco Targets Ther 12:9201-9213 (2019). PubMed: 31807004
