Anti-Wnt16 抗体 [EPR22889-129] - BSA and Azide free (ab272322)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22889-129] to Wnt16 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Human
製品の概要
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製品名
Anti-Wnt16 antibody [EPR22889-129] - BSA and Azide free
Wnt16 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22889-129] to Wnt16 - BSA and Azide free -
由来種
Rabbit -
Tested Applications & Species
Application Species IP Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: IMR-90 and HEK-293 whole cell lysates. IP: HEK-293 whole cell lysate.
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特記事項
ab272322 is the carrier-free version of ab269278. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22889-129 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
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IP |
Human
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アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
組織特異性
Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 51384 Human
- Omim: 606267 Human
- SwissProt: Q9UBV4 Human
- Unigene: 272375 Human
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別名
- Protein Wnt 16 precursor antibody
- Protein Wnt-16 antibody
- Wingless type MMTV integration site family member 16 antibody
see all
画像
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Wnt16 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate using ab269278 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab269278 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used as the secondary anyibody at 1/5000 dilution.
Lane 1: HEK-293 whole cell lysate (input)
Lane 2: ab269278 IP in HEK-293 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269278 in HEK-293 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 mintues
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269278).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab272322 は論文での使用が確認できていません。