Anti-Wnt10a 抗体 (ab106522)
Key features and details
- Rabbit polyclonal to Wnt10a
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Wnt10a antibody
Wnt10a 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Wnt10a -
由来種
Rabbit -
特異性
Despite the high homology of Wnt10a to Wnt10b, ab106522 will not cross-react with Wnt10b. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
A 14 amino acid synthetic peptide from near the C terminus of Human Wnt10a (UniProt Q9GZT5).
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ポジティブ･コントロール
- WB: RAW264.7 cell lysate. IHC-P: Human and mouse skeletal muscle tissue; Rat bladder tissue. ICC/IF: Human skeletal muscle cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106522の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
Not validated in human. |
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| IHC-P | (1) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa. Not validated in human. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. -
関連疾患
Defects in WNT10A are a cause of ectodermal dysplasia anhidrotic (EDA) [MIM:224900]; also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands. Note=Most patients carrying WNT10A mutations present with sweating anomalies. However, comparison with EDA cases harboring mutations in the ectodysplasin pathway identifies some phenotypic differences. Dermatological features (anomalies of hair and sweat glands) are less severe in patients carrying WNT10A mutations and facial dysmorphism can be absent. The dental phenotype consists in microdontia, whereas teeth agenesis is more frequent in patients carrying mutations in the ectodysplasin pathway.
Defects in WNT10A are a cause of odonto-onycho-dermal dysplasia (OODD) [MIM:257980]. OODD is a rare autosomal recessive ectodermal dysplasia in which the presenting phenotype is dry hair, severe hypodontia, smooth tongue with marked reduction of fungiform and filiform papillae, onychodysplasia, keratoderma and hyperhidrosis of palms and soles, and hyperkeratosis of the skin.
Defects in WNT10A are a cause of Schopf-Schulz-Passarge syndrome (SSPS) [MIM:224750]. SSPS is rare ectodermal dysplasia, characterized chiefly by cysts of the eyelid margins, palmoplantar keratoderma, hypodontia, hypotrichosis and nail dystrophy. Multiple eyelid apocrine hidrocystomas are the hallmark of this condition, although they usually appear in adulthood. The concomitant presence of eccrine syringofibroadenoma in most patients and of other adnexal skin tumors in some affected subjects indicates that Schopf-Schulz-Passarge syndrome is a genodermatosis with skin appendage neoplasms. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 80326 Human
- Entrez Gene: 22409 Mouse
- Omim: 606268 Human
- SwissProt: Q9GZT5 Human
- SwissProt: P70701 Mouse
- Unigene: 121540 Human
- Unigene: 5130 Mouse
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別名
- FLJ14301 antibody
- OODD antibody
- Protein Wnt-10a antibody
see all
画像
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Western blot - Anti-Wnt10a antibody (ab106522)Lane 1 : Anti-Wnt10a antibody (ab106522) at 1 µg/ml
Lane 2 : Anti-Wnt10a antibody (ab106522) at 2 µg/ml
All lanes : RAW264.7 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 46 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (ab106522)Paraffin-embedded mouse skeletal muscle tissue stained for Wnt10a using ab106522 at 5 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (ab106522)Paraffin-embedded human skeletal muscle tissue stained for Wnt10a using ab106522 at 10 µg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt10a antibody (ab106522)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of rat bladder tissue, staining Wnt10a with ab106522.
Tissue was fixed with formalin and blocked with 5000 µg/ml BSA for 30 minutes at 22°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in BSA) for 1 hour at 22°C. An AlexaFluor®555-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-Wnt10a antibody (ab106522)Immunofluorescence of Wnt10a in Human Skeletal Muscle cells using ab106522 at 20 ug/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab106522 は 10 報の論文で使用されています。
- Zhu L et al. WNT/ß-catenin pathway activation via Wnt1 overexpression and Axin1 downregulation correlates with cadherin-catenin complex disruption and increased lymph node involvement in micropapillary-predominant lung adenocarcinoma. J Thorac Dis 12:5906-5915 (2020). PubMed: 33209423
- Piotrowska Z et al. Comparative Assessment of the WNT/ß-Catenin Pathway, CacyBP/SIP, and the Immunoproteasome Subunit LMP7 in Various Histological Types of Renal Cell Carcinoma. Front Oncol 10:566637 (2020). PubMed: 33330038
- Zhou H et al. MicroRNA-361-3p Inhibit the Progression of Lymphoma by the Wnt/ß-Catenin Signaling Pathway. Cancer Manag Res 12:12375-12384 (2020). PubMed: 33299350
- He L et al. Parenchymal and stromal tissue regeneration of tooth organ by pivotal signals reinstated in decellularized matrix. Nat Mater 18:627-637 (2019). PubMed: 31114073
- Zhong JM et al. Dexmedetomidine Reduces Diabetic Neuropathy Pain in Rats through the Wnt 10a/ß-Catenin Signaling Pathway. Biomed Res Int 2018:9043628 (2018). PubMed: 30622965
- Cheng JM et al. Role of WNT signaling in epididymal sperm maturation. J Assist Reprod Genet 35:229-236 (2018). PubMed: 29152689
- Zhao B et al. Hyperbaric oxygen attenuates neuropathic pain and reverses inflammatory signaling likely via the Kindlin-1/Wnt-10a signaling pathway in the chronic pain injury model in rats. J Headache Pain 18:1 (2017). IHC ; Rat . PubMed: 28058534
- Sun X et al. Colorectal cancer cells suppress CD4+ T cells immunity through canonical Wnt signaling. Oncotarget 8:15168-15181 (2017). PubMed: 28147310
- Chen B et al. Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/ß-catenin signaling pathway activation. Int J Mol Med 38:1531-1540 (2016). PubMed: 28026000
- Long A et al. WNT10A promotes an invasive and self-renewing phenotype in esophageal squamous cell carcinoma. Carcinogenesis 36:598-606 (2015). PubMed: 25795715
