Withaferin A from Withania somnifera (ab120644)
Key features and details
- Naturally occurring withanolide/steroidal lactone with anticancer properties
- CAS Number: 5119-48-2
- Purity: > 98%
Soluble in ethanol
- Form / State: Solid
- Source: Withania somnifera
製品の概要
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製品名
Withaferin A from Withania somnifera -
製品の詳細
Naturally occurring withanolide/steroidal lactone with anticancer properties -
別名
- Withaferine A
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生理活性の詳細
Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
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精製度
> 98% -
CAS 番号
5119-48-2 -
構造式
製品の特性
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体系名
(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
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分子量
470.60 -
分子式
C28H38O6 -
保存方法
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in ethanol
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Withania somnifera
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120644の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120644, Withaferin A from Withania somnifera
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ab7973 staining Bcl2 in HEK 293 cells treated with withaferin A from Withania somnifera (ab120644), by ICC/IF. Decrease in Bcl2 expression with increased concentration of withaferin A from Withania somnifera, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120644 (withaferin A from Withania somnifera) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7973 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab120644 は 2 報の論文で使用されています。
- Shiota T et al. C9orf72-Derived Proline:Arginine Poly-Dipeptides Modulate Cytoskeleton and Mechanical Stress Response. Front Cell Dev Biol 10:750829 (2022). PubMed: 35399536
- Cortese M et al. Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies. Cell Host Microbe 28:853-866.e5 (2020). PubMed: 33245857