Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS Cell)
Permeabilization
Yes - 0.2% NP-40
Specification
U2OS Cell
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1% BSA in PBS
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Mouse IgG (H+L) (A-21202)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
U2OS cells incubated in complete media (control) or Earls balanced salt solution (EBSS) for 2h to induce autophagy.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Michael Munson
Verified customer
投稿 Oct 02 2018