Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (sheep uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citric acid,pH 6.0, 0,05% Tween 20
Permeabilization
Yes - Triton-X100 0.2%
Specification
sheep uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: Blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 29 2020