WI-38 whole cell lysate (ab3960)

Submit a review Q&A (1)References (1)

製品の概要

  • 製品名

    WI-38 whole cell lysate

  • 特記事項

    Cell line: WI-38 (Human lung fibroblast, normal).
    Growth media: Minimum essential medium (Eagle) with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, and 10% FBS.


    WI-38 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • アプリケーション

    適用あり: WBmore details

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Constituents: 12.5% Glycerol (glycerin, glycerine), 1% Sodium lauryl sulfate, 0.01% Bromophenol blue, 0.79% Tris HCl, 0.77% DTT
  • Concentration information loading...

  • ライゼート 備考

    WI-38 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3960の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent dilution. Ready to load; 10 - 20 µg/lane is recommended for mini gel.
特記事項
WB
Use at an assay dependent dilution. Ready to load; 10 - 20 µg/lane is recommended for mini gel.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab3960 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3960 は 1 報の論文で使用されています。

  • Weitzel LR  et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat 124:297-305 (2010). PubMed: 20087651

レビューと Q&A

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Question

I am interested in purchasing a fibroblast whole cell lysate product from you company to test a new procollagen probe my lab developed.

I noticed that you sell several kinds of fibroblast lysate products:ab7179, ab3960, ab3874, ab7901...

I wanna know if these products have a high procollagen (˜150KDa) content. Have you tested that? Or do you know if there have been any existing reports in which these products have been used for procollagen detection? This information is important for me to make the decision.

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Answer

Thank you for contacting Abcam and for your interest in our whole cell lysates.

Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.

If you are interested in determining which lysates will contain the protein that you need, I would recommend a search of the literature. Unfortunately, we are not experts in this field and would not want to recommend a product that is not suitable for your experiments.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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