WI-38 nuclear extract lysate (forskolin stimulated) (ab14912)
製品の概要
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製品名
WI-38 nuclear extract lysate (forskolin stimulated)
WI 38 ライゼート 製品一覧 -
特記事項
The WI-38 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM). The protein content has been determined by a Bradford-based assay.
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.0002% Sodium molybdate, 0.216% Beta glycerophosphate, 0.018% Sodium orthovanadate, 0.042% Sodium fluoride, 0.015% DTT, 0.48% HEPES, 0.003% EDTA, 20% Glycerol, 2.34% Sodium chloride
With Protease inhibitor -
Concentration information loading... -
研究分野
画像
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Functional Studies - WI-38 nuclear extract lysate (forskolin stimulated) (ab14912)
Each lot has been tested for CREB activation by using TransAM™ pCREB Kits. The signal intensity for CREB activation in each lot is compared to the signal intensity obtained with extracts from unstimulated WI-38 VA13 cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 3. This ratio may vary depending on the basal level of CREB activation in a given cell type.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab14912 は論文での使用が確認できていません。
