Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (lymphocytes)
Specification
lymphocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: cell signaling
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ecl
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
Nothing showed up, same sample shows bands with another WRN antibody reviewed (ab133618).
Abcam response
Thank you for your valuable feedback. your data suggests that ab17987 may not detect Werner's syndrome helicase (WRN) in human lymphocytes under these experimental conditions. We welcome further customer feedback.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 05 2014