Anti-Wee1 抗体 (ab137377)
Key features and details
- Rabbit polyclonal to Wee1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Wee1 antibody
Wee1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Wee1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment, corresponding to a region within internal amino acids 274 and 506 of Human Wee1.
-
ポジティブ・コントロール
- WB: Jurkat, Raji, NCI-H929 whole cell lysate. IHC:CL1-0 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137377の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 72 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
|
特記事項 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 72 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
ターゲット情報
-
機能
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated during M and G1 phases. Also autophosphorylated.
Ubiquitinated and degraded at the onset of G2/M phase. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 7465 Human
- Omim: 193525 Human
- SwissProt: P30291 Human
- Unigene: 249441 Human
-
別名
- DKFZp686I18166 antibody
- EC 2.7.10.2 antibody
- FLJ16446 antibody
see all
画像
-
All lanes : Anti-Wee1 antibody (ab137377) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 72 kDa7.5% SDS-PAGE
-
Immunohistochemical analysis of paraffin embedded CL1-0 (Lung adenocarcinoma) xenograft tissue stained Wee1 using ab137377 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab137377 は 8 報の論文で使用されています。
- Sun L et al. Verbascoside enhances radiosensitivity of hepatocellular carcinoma cells through regulating miR-101-3p/Wee1 axis. Drug Dev Res 83:891-899 (2022). PubMed: 35080031
- Du R et al. Knockdown of lncRNA X inactive specific transcript (XIST) radiosensitizes non-small cell lung cancer (NSCLC) cells through regulation of miR-16-5p/WEE1 G2 checkpoint kinase (WEE1) axis. Int J Immunopathol Pharmacol 35:2058738420966087 (2021). PubMed: 33583218
- Tampakakis E et al. Heart neurons use clock genes to control myocyte proliferation. Sci Adv 7:eabh4181 (2021). PubMed: 34851661
- Jin H et al. A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. Sci Adv 6:eaba3418 (2020). PubMed: 32426509
- Zhang N et al. Ropivacaine Inhibits the Growth, Migration and Invasion of Gastric Cancer Through Attenuation of WEE1 and PI3K/AKT Signaling via miR-520a-3p. Onco Targets Ther 13:5309-5321 (2020). PubMed: 32606749
- Lee WH et al. DNA primase polypeptide 1 (PRIM1) involves in estrogen-induced breast cancer formation through activation of the G2/M cell cycle checkpoint. Int J Cancer 144:615-630 (2019). PubMed: 30097999
- Ho TL et al. The KRAB Zinc Finger Protein Roma/Zfp157 Is a Critical Regulator of Cell-Cycle Progression and Genomic Stability. Cell Rep N/A:N/A (2016). WB . PubMed: 27149840
- Puente BN et al. The oxygen-rich postnatal environment induces cardiomyocyte cell-cycle arrest through DNA damage response. Cell 157:565-79 (2014). PubMed: 24766806