Anti-WDR19 抗体 [EPR24915-11] - BSA and Azide free (ab283709)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24915-11] to WDR19 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-WDR19 antibody [EPR24915-11] - BSA and Azide free
WDR19 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24915-11] to WDR19 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293, HeLa and mIMCD3 whole cell lysate; Human epididymis, Mouse testis and Rat testis tissue lysate. IP: mIMCD3 cell.
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特記事項
ab283709 is the carrier-free version of ab270970.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24915-11 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab283709の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. |
ターゲット情報
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機能
Component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport (PubMed:20889716). Involved in cilia function and/or assembly (By similarity). Associates with the BBSome complex to mediate ciliary transport. -
組織特異性
Some isoforms are tissue-specific. Highly expressed in the prostate. Lower expression in the cerebellum, pituitary gland, fetal lung, and pancreas. In normal prostate, expressed in both basal and luminal epithelial cells. No expression detected in fibromuscular stromal cells, endothelial cells, or infiltrating lymphocytes. Uniformed expression in prostate adenocarcinoma cells. -
関連疾患
Cranioectodermal dysplasia 4
Short-rib thoracic dysplasia 5 with or without polydactyly
Nephronophthisis 13
Senior-Loken syndrome 8 -
配列類似性
Contains 6 TPR repeats.
Contains 6 WD repeats. -
発生段階
Expressed in fetal lung. -
細胞内局在
Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body. Cell projection, cilium, photoreceptor outer segment. Localizes to photoreceptor connecting cilia, to the base of motile cilia in brain ependymal cells and to the base of and along primary cilia in kidney cells. - Information by UniProt
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参照データベース
- Entrez Gene: 57728 Human
- Entrez Gene: 213081 Mouse
- Entrez Gene: 305349 Rat
- Omim: 608151 Human
- SwissProt: Q8NEZ3 Human
- SwissProt: Q3UGF1 Mouse
- Unigene: 438482 Human
- Unigene: 127550 Mouse
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別名
- DYF 2 antibody
- DYF2 antibody
- FLJ23127 antibody
see all
画像
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All lanes : Anti-WDR19 antibody [EPR24915-11] (ab270970) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : mIMCD3 (mouse inner medullary collecting duct epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 151 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab270970, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 26 seconds.
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This data was developed using ab270970, the same antibody clone in a different buffer formulation.
WDR19 was immunoprecipitated from 0.35 mg mIMCD3 (mouse inner medullary collecting duct epithelial cell), whole cell lysate 10 ug with ab270970 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270970 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: mIMCD3 (mouse inner medullary collecting duct epithelial cell), whole cell lysate 10 ug
Lane 2: ab270970 IP in mIMCD3 whole cell lysate
Lane 3:Rabbit monoclonal IgG (ab172730) instead of ab270970 in mIMCD3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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All lanes : Anti-WDR19 antibody [EPR24915-11] (ab270970) at 1/1000 dilution
Lane 1 : Human epididymis tissue lysate
Lane 2 : Human spleen tissue lysate
Lane 3 : Mouse testis tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat testis tissue lysate
Lane 6 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 151 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This data was developed using ab270970, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Samples are non-boiled as boiling may cause protein aggregates.
Low expression: Spleen (PMID: 12906858)
Exposure time: Lane 1-2: 26 seconds; Lane 3-6: 103 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab283709 は論文での使用が確認できていません。