Anti-WDR13 抗体 (ab272543)
Key features and details
- Rabbit polyclonal to WDR13
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WDR13 antibody
WDR13 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR13 -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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免疫原
Recombinant fragment corresponding to Human WDR13 aa 100-250.
Database link: Q9H1Z4 -
ポジティブ･コントロール
- WB: Human cell line RT-4 IHC-P: Human fallopian tissue ICC/IF: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Chimpanzee
Orangutan
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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組織特異性
Widely expressed. -
配列類似性
Contains 5 WD repeats. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 64743 Human
- Entrez Gene: 73447 Mouse
- Omim: 300512 Human
- SwissProt: Q9H1Z4 Human
- SwissProt: Q91V09 Mouse
- Unigene: 521973 Human
- Unigene: 284162 Mouse
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別名
- DKFZp779C2057 antibody
- FLJ20563 antibody
- located at OATL1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR13 antibody (ab272543)
Paraffin-embedded formalin-fixed Human fallopian tissue stained for WDR13 using ab272543 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-WDR13 antibody (ab272543)Lane 2 : Anti-WDR13 antibody (ab272543) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : Human cell line RT-4 -
Immunocytochemistry/ Immunofluorescence - Anti-WDR13 antibody (ab272543)PFA fixed, Triton X-100 permeablized human cell line A-431 labeling WDR13 (green) using ab272543 at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDR13 antibody (ab272543)Paraffin-embedded formalin-fixed Human tonsil tissue stained for WDR13 using ab272543 1/500 dilution in immunohistochemical analysis. Shows low expression as expected.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272543 は論文での使用が確認できていません。