Anti-WDR1 抗体 [EPR8793] (ab173574)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8793] to WDR1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-WDR1 antibody [EPR8793]
WDR1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8793] to WDR1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow -
免疫原
Synthetic peptide within Human WDR1 aa 300-400. The exact sequence is proprietary.
Database link: O75083 -
ポジティブ・コントロール
- WB: Human heart tissue lysate. Jurkat, Raji, HT-1080 and U87-MG cell lysates. IHC: Human skeletal muscle tissue and endometrial carcinoma tissue. ICC/IF: Raji cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR8793 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173574の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
- Information by UniProt
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参照データベース
- Entrez Gene: 422842 Chicken
- Entrez Gene: 533223 Cow
- Entrez Gene: 9948 Human
- Entrez Gene: 22388 Mouse
- Entrez Gene: 360950 Rat
- Omim: 604734 Human
- SwissProt: O93277 Chicken
- SwissProt: Q2KJH4 Cow
see all -
別名
- Actin interacting protein 1 antibody
- Actin-interacting protein 1 antibody
- AIP1 antibody
see all
画像
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Anti-PDK2 antibody [EPR1948Y] (ab68164) at 1/200 dilution + Human heart tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (HRP), with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 66 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBuffer concentration: 5% NFDM /TBST.
For human samples, please use lower antibody dilution or upload more of the protein.
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All lanes : Anti-WDR1 antibody [EPR8793] (ab173574) at 1/2000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 66 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBuffer concentration: 5% NFDM /TBST
We are unsure as to the identity of the extra band below 75kDa.
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Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling WDR1 with ab173574 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling WDR1 with ab173574 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescence analysis of Raji cells labeling WDR1 with ab173574 at a 1/50 dilution (green). DAPI nuclear staining (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab173574 は 4 報の論文で使用されています。
- Yao W et al. TNK2-AS1 upregulated by YY1 boosts the course of osteosarcoma through targeting miR-4319/WDR1. Cancer Sci 112:893-905 (2021). PubMed: 33164271
- Iqbal J et al. Selenium positively affects the proteome of 3 × Tg-AD mice cortex by altering the expression of various key proteins: unveiling the mechanistic role of selenium in AD prevention. J Neurosci Res 96:1798-1815 (2018). PubMed: 30054946
- López-Coral A et al. A Serine/Threonine Kinase 16-Based Phospho-Proteomics Screen Identifies WD Repeat Protein-1 As A Regulator Of Constitutive Secretion. Sci Rep 8:13049 (2018). PubMed: 30158666
- Hong F et al. Mapping the Interactome of a Major Mammalian Endoplasmic Reticulum Heat Shock Protein 90. PLoS One 12:e0169260 (2017). PubMed: 28056051