Western blot abreview for Anti-VSV-G tag antibody

Good
Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG HRP Promega
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 31, 54 kDa Non-specific: between 49 and 52 kDa
Positive control
strain expressing recombinant protein (vector expression)
Negative control
strain without expression of recombinant protein (without vector)

Additional data

Additional Notes
52kDa non-specific band corresponds to tubulin (alpha, beta or gamma)
Abcam response
Thank you for your very interesting feedback. This antibody has only been tested for IF application and we are very glad to hear that you have used it successfully for Western blot.

Thank you for your time, your effort and your understanding! We are looking forward to receiving your new review.

Mr. Chesneau Laurent

Verified customer

投稿 Sep 02 2005

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