製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to VSV-G tag
  • 由来種

    Rabbit
  • 特異性

    This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
  • アプリケーション

    適用あり: ICC, IHC-Fr, WB, IP, ICC/IFmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    A synthetic peptide corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence:

    YTDIEMNRLGK

    .

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1874 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100.
IHC-Fr Use at an assay dependent concentration. PubMed: 15662032
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF 1/200.

ターゲット情報

  • 関連性

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 別名

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

画像

  • ab1874 detecting bound HS scFv antibody in rat lung tissue by Immunohistochemistry (Frozen sections).
    Lungs from Sprague-Dawley rats were harvested at embryonic days 17.5 of gestation and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4. The lungs were washed in PBS, cryoprotected with 20% (w/v) sucrose in PBS, and gelatin-embedded (7.5% (w/v) gelatin, 15% (w/v) sucrose in PBS) before being covered in Cryo-M-Bed and snap frozen at -40 °C. The lung sections were cut at 8 µm and mounted onto chrome alum gel slides for storage at -40 °C. Tissue sections were blocked with 10% (v/v) goat serum in PBS and incubated overnight at 4 °C with HS scFv antibody, diluted 1/5 in 1% (v/v) goat serum in PBS. Bound antibody was detected with ab1874 diluted 1/200 in 1% (v/v) goat serum in PBS, followed by fluorescein isothiocyanate-conjugated goat anti-rabbit IgG.

    aw, airway; me, mesenchyme; bm, basement membrane.

参考文献

This product has been referenced in:

  • Dutta P  et al. A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease. Neurosci Lett 673:12-18 (2018). ICC/IF . Read more (PubMed: 29496607) »
  • Ni YX  et al. A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating. Cell Discov 4:65 (2018). Read more (PubMed: 30603101) »
See all 13 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

Filter by Ratings

1-2 of 2 Abreviews

Application
Immunocytochemistry
Sample
Saccharomyces cerevisiae Cell
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%

Mr. Chesneau Laurent

Verified customer

投稿 Sep 19 2005

Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%

Mr. Chesneau Laurent

Verified customer

投稿 Sep 02 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録