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  1. Link

    vsv-g-tag-antibody-ab1874.pdf

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Tags & Cell Markers Epitope Tags Other Tags VSV-G Tag
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Anti-VSV-G tag 抗体 (ab1874)

  • Datasheet
  • SDS
Reviews (2)Q&A (4)References (16)

Product price, shipping and contact information

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出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Rabbit polyclonal to VSV-G tag
  • Suitable for: ICC, WB, IP, ICC/IF
  • Reacts with: Species independent
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-VSV-G tag antibody [EPR12997] (ab183497)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-VSV-G tag antibody
    VSV-G tag 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to VSV-G tag
  • 由来種

    Rabbit
  • 特異性

    This product has been assayed by ELISA against 0.1 µg of the immunizing peptide. A 1/8000 to 1/32000 dilution of the antibody is recommended for this assay.
  • アプリケーション

    適用あり: ICC, WB, IP, ICC/IFmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    A synthetic peptide corresponding to aa 501-511 of vesicular stomatitis virus glycoprotein (VSV-G). This vector is a pseudo lentiviral vector. Peptide sequence:

    YTDIEMNRLGK

    .
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • VSV-G Tag

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab1874の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (1)
1/100.
WB (1)
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
1/200.
特記事項
ICC
1/100.
WB
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
1/200.

ターゲット情報

  • 関連性

    Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV-g) contains a domain in its extracellular membrane proximal stem that appears to be needed for efficient VSV budding.
  • 別名

    • Vesicular stomatitis virus glycoprotein tag antibody
    • VSV epitope tag antibody
    • VSV tag antibody
    • VSV-G epitope tag antibody
    • VSVG antibody
    • vsvg tag antibody
    see all

プロトコール

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (16)

ab1874 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab1874 は 16 報の論文で使用されています。

  • Voss TD  et al. Ubiquitination and the proteasome rather than caspase-3-mediated C-terminal cleavage are involved in the EAAT2 degradation by staurosporine-induced cellular stress. J Neurochem 157:1284-1299 (2021). PubMed: 33180957
  • Xu J  et al. The RNA helicase DDX5 promotes viral infection via regulating N6-methyladenosine levels on the DHX58 and NF?B transcripts to dampen antiviral innate immunity. PLoS Pathog 17:e1009530 (2021). PubMed: 33909701
  • Cui S  et al. Nuclear cGAS Functions Non-canonically to Enhance Antiviral Immunity via Recruiting Methyltransferase Prmt5. Cell Rep 33:108490 (2020). PubMed: 33296647
  • Ni YX  et al. A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating. Cell Discov 4:65 (2018). PubMed: 30603101
  • Dutta P  et al. A novel modelling mechanism of PAEL receptor and GABARAPL2 interaction involved in Parkinson's disease. Neurosci Lett 673:12-18 (2018). ICC/IF . PubMed: 29496607
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Immunocytochemistry abreview for Anti-VSV-G tag antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Saccharomyces cerevisiae Cell
Fixative
Formaldehyde
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1%
Read More

Mr. Chesneau Laurent

Verified customer

投稿 Sep 19 2005

Western blot abreview for Anti-VSV-G tag antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Saccharomyces cerevisiae Cell lysate - whole cell
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Read More

Mr. Chesneau Laurent

Verified customer

投稿 Sep 02 2005

Question

Hello,



I am looking for an antibody for detecting whether my cells are expressing VSV-G (Western blot). I have found several ab on-line, but do you also have cell lysate as a positive control?



Best regards

Read More

Abcam community

Verified customer

Asked on Mar 07 2012

Answer

Thank you for your enquiry and your interest in our products.

We have a few antibodies which detect VSV-G and are suitable for Western applications. Some of them are unconjugated so it is essential to apply a compatible secondary antibody for detection:

ab1874: https://www.abcam.com/vsv-g-tag-antibody-ab1874.html

ab27026: https://www.abcam.com/vsv-g-tag-antibody-ab27026.html

ab50549: https://www.abcam.com/vsv-g-tag-antibody-p5d4-ab50549.html

Some others are HRP-labelled so can be applied without any secondary antibodies:

ab3556: https://www.abcam.com/vsv-g-tag-antibody-hrp-ab3556.html

ab49610: https://www.abcam.com/vsv-g-tag-antibody-p5d4-hrp-ab49610.html

Currently, we do not sel lpositive control cell lysate for VSV-G. As long as the cells you are planning to use express the tag sequence: YTDIEMNRLGK; these antibodies should work fine.

Read More

Abcam Scientific Support

Answered on Mar 07 2012

Question

I would like to know if this antibody (AB1874, VSV-G tag) is able to bind to recombinant lentiviral vectors pseudotyped with the VSV-G enveloppe.

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

The antibody has not been tested in this way so we do not know it it will bind.

Read More

Asdf Edo

Abcam Scientific Support

Answered on Oct 13 2003

Question

I would like to know if this antibody (AB1874, VSV-G tag) is able to bind to recombinant lentiviral vectors pseudotyped with the VSV-G enveloppe.

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

Read More

Asdf Edo

Abcam Scientific Support

Answered on Oct 13 2003

Question

Phone call requesting information on antibodies directed against the VSV-G tag for use in IHC.

Read More

Abcam community

Verified customer

Asked on Oct 09 2012

Answer

Thank you for contacting us and your interest in our products.

As discussed over the phone, I have looked into the antibodies we have which are directed against the VSV-G tag. As you have seen, the reference where ab1874 was used in the staining of frozen rat lung tissue were not used to detect the epitope directly as part of the tissue. It was used to detect and antibody directed against heparan sulfate which contained the tag. The antibody was therefore used more like a secondary antibody.

Unfortunately, we do not have any antibodies where the VSV-G epitope has been detected for IHC as yet. This does not mean that the antibodies would not work in this application, simply that we have not tested it ourselves and would not be able to guarantee its performance.

I am however able to offer you a testing discount if you would like to try one of our antibodies. This offer means that if you purchase an antibody such as the rabbit polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab1874.html or chicken polyclonal https://www.abcam.com/VSV-G-tag-antibody-ab27026.html as normal, test the antibody with your mouse samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the value of the antibody tested off any future purchase with Abcam. More information on this offer can be found from the following link:

https://www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of the antibody to be tested.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Abcam Scientific Support

Answered on Oct 09 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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