Anti-VR1 抗体 (ab3487)

Reviews (2)Q&A (2)References (10)

Key features and details

  • Rabbit polyclonal to VR1
  • Suitable for: IHC-Fr, IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-VR1 antibody
    VR1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to VR1

  • 由来種

    Rabbit

  • 特異性

    Immunohistochemical staining of VR1 in human glabrous skin using this antibody results in the staining of epidermal and dermal cells, as well as free nerve fibers and Merkel cells. Immunofluorescent staining of VR1 in human prostatic urethra results in fibrous staining within the epithelial cells.

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WB, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human VR1 aa 7-21.
    Sequence:

    TDLGAAADPLQKDTC


    (Peptide available as ab5894)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 一次抗体 備考

    Vanilloid Receptor 1 (VR1), also known as capsaicin receptor, responds to both capsaicin, the pungent ingredient in hot peppers, and thermal stimuli within the noxious temperature range. This receptor is structurally related to the TRP family of ion channels and is predicted to contain 6 trans-membrane domains, a P-loop membrane domain and 3 ankyrin repeat domains. VR1 is highly permeable to divalent cations, including calcium and magnesium, as well as the monovalent cations sodium and potassium. Expression of VR1 appears to be limited to neurons within the sensory ganglia.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.

  • 組織特異性

    Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

  • 配列類似性

    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
    Contains 6 ANK repeats.

  • ドメイン

    The association domain (AD) is necessary for self-association.

  • 翻訳後修飾

    Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.

  • 細胞内局在

    Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Capsaicin receptor antibody
      • DKFZp434K0220 antibody
      • osm 9 like TRP channel 1 antibody
      • Osm-9-like TRP channel 1 antibody
      • OTRPC1 antibody
      • Transient receptor potential cation channel subfamily V member 1 antibody
      • TRPV 1 antibody
      • Trpv1 antibody
      • TRPV1_HUMAN antibody
      • Vanilloid receptor 1 antibody
      • Vanilloid receptor subtype 1 antibody
      • VR 1 antibody
      • VR1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VR1 antibody (ab3487)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VR1 antibody (ab3487)

      Immunolocalization of VR1 in human glabrous skin using ab3487.

    • Immunohistochemistry (Frozen sections) - Anti-VR1 antibody (ab3487)
      Immunohistochemistry (Frozen sections) - Anti-VR1 antibody (ab3487)

      Immunohistochemistry (frozen sections) analysis of human skin tissue sections labeling VR1 (red) with ab3487 at 1/250. A biotin-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

    参考文献 (10)

    ab3487 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3487 は 10 報の論文で使用されています。

    • Jian T  et al. Flavonoids isolated from loquat (Eriobotrya japonica) leaves inhibit oxidative stress and inflammation induced by cigarette smoke in COPD mice: the role of TRPV1 signaling pathways. Food Funct 11:3516-3526 (2020). PubMed: 32253400
    • Baker C  et al. Natural product-drug conjugates for modulation of TRPV1-expressing tumors. Bioorg Med Chem 27:2531-2536 (2019). PubMed: 30885569
    • Hashimoto T  et al. Pathophysiological mechanisms of itch in bullous pemphigoid. J Am Acad Dermatol N/A:N/A (2019). PubMed: 31351883
    • Zhang D  et al. 125I radiation downregulates TRPV1 expression through miR-1246 in neuroblastoma cells. Oncol Rep 42:243-252 (2019). PubMed: 31059092
    • Rice FL  et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
    • Nattkemper LA  et al. The Genetics of Chronic Itch: Gene Expression in the Skin of Patients with Atopic Dermatitis and Psoriasis with Severe Itch. J Invest Dermatol 138:1311-1317 (2018). PubMed: 29317264
    • Ives SJ  et al. TRPV1 channels in human skeletal muscle feed arteries: implications for vascular function. Exp Physiol 102:1245-1258 (2017). PubMed: 28681979
    • Deering-Rice CE  et al. Characterization of Transient Receptor Potential Vanilloid-1 (TRPV1) Variant Activation by Coal Fly Ash Particles and Associations with Altered Transient Receptor Potential Ankyrin-1 (TRPA1) Expression and Asthma. J Biol Chem 291:24866-24879 (2016). PubMed: 27758864
    • Deering-Rice CE  et al. Transient receptor potential vanilloid-1 (TRPV1) is a mediator of lung toxicity for coal fly ash particulate material. Mol Pharmacol 81:411-9 (2012). WB . PubMed: 22155782
    • Denda S  et al. Glycolic acid induces keratinocyte proliferation in a skin equivalent model via TRPV1 activation. J Dermatol Sci 57:108-13 (2010). IHC-Fr ; Human . PubMed: 20060270

    レビューと Q&A

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    1-4 of 4 Abreviews or Q&A

    Question

    I tried to test the indicate protocol : https://www.abcam.com/index.html?datasheet=3487&tab=abreviews&intabreviewid=26192 : test fixation methanol, over night, goat serum saturation, permeabilization triton X100

    But without success !

    To answer to your question (Can you confirm that the other reactives (e.g. the secondary antibody) do work fine when used with other primary rabbit antibodies?) , yes, we use this secondary antibody frequently and it work very well.

    Please can you give me a solution, my client want a VR1 labelling on human skin !

    Thanks a lot

    Regards.

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    Answer

    Thank your email. I am very sorry that the suggestions did not helpto improve the results. I must therefore conclude, that the vial you received must have been damaged - maybe during storage or transport. I would be pleased to replace this vial for you, by either a credit/refund or a replacement vial. Please let me know which optionyou prefer andwhether you would like to accept either a new vial of ab3487 or another alternative antibody from our catalog?
    I apologize for the inconvenience. Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    Inquiry: Mr or Mrs I tried the antibody ab3487 (anti-VR1) on frozen section of skin : - fixed in aceton/methanol - saturation in milk - antibody dilution : 1/1000 and 1/100, 1 hour at RT - secondary antibody GAR alexa fluor 488, 1 hour at RT - wash in PBS-tween 20. It didn't work. Do you have a specific protocol for this marker ? Could you give me the conditions please ? Thanks a lot. Regards

    Read More
    Answer

    Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

    We do not have a specific protocol for this antibody. We expect this antibody to work with a general IHC protocol after the classic optimisation steps. Looking at the information you provided, I would like to offerthe followingsuggestions to help optimize the results from ab3487. I would also appreciate if you can confirm some further details:

    1.) Can you please confirm of which species the skin sections usedare coming from. We have tested this antibody so far only on human tissue. It might reacts with other species, however we need to test this first.

    2.) I can recommend to incubate the antibody overnight at 4°C. This can increase the staining intensity.

    3.) I can also recommend to test different blocking solutions. This can significantly improve the staining. I can recommend to test BSA and serum instead of milk. As serum you would use serum of the species of the secondary antibody (goat?). The researcher having submitted the feedback on this antibody, has used for example 10% serum. See his Abreview: https://www.abcam.com/index.html?datasheet=3487&tab=abreviews&intabreviewid=26192

    4.) Can you confirm that the other reactives (e.g. the secondary antibody) do work fine when used with other primary rabbit antibodies?

    5.) Have you tested other fixation methods as well, such as Methanol only or Paraformaldehyde? The user mentionned above, had used succesfully Methanol only, whereas the lab has tested the antibody also in paraformaldhyde fixation obtaining good results.

    I hope these suggestions will help to identify the problem. Should the suggestions not improve the results, please do let me know.

    In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

    I hope this information is helpful, and I thank you for your cooperation.

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    Immunohistochemistry (Frozen sections) abreview for Anti-VR1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin, 3D skin model)
    Specification
    skin, 3D skin model
    Fixative
    Methanol
    Permeabilization
    Yes - 0.4% Triton X100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 21 2011

    Immunohistochemistry - Free Floating abreview for Anti-VR1 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 10 2011

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