Anti-VPS26 抗体 [EPR13456] (ab181352)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13456] to VPS26
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VPS26 antibody [EPR13456]
VPS26 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13456] to VPS26 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IPmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, HeLa, 293T, and A431 cell lysates; HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13456 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
|
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
配列類似性
Belongs to the VPS26 family. -
細胞内局在
Cytoplasm. Endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9559 Human
- Entrez Gene: 30930 Mouse
- Entrez Gene: 361846 Rat
- Omim: 605506 Human
- SwissProt: O75436 Human
- SwissProt: P40336 Mouse
- SwissProt: Q6AY86 Rat
- Unigene: 499925 Human
see all -
別名
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181352 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab181352 was shown to specifically react with VPS26 when VPS26 knockout samples were used. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab181352 and ab18058 (loading control to Vinculin) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ( ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Immunofluorescent analysis of HeLa cells labeling VPS26 with ab181352 at 1/100 dilution (red). DAPI nuclear staining (blue).
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All lanes : Anti-VPS26 antibody [EPR13456] (ab181352) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 38 kDa -
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate pulling down VPS26 using ab181352 at 1/10 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (13)
ab181352 は 13 報の論文で使用されています。
- Courtellemont T et al. CROP: a retromer-PROPPIN complex mediating membrane fission in the endo-lysosomal system. EMBO J 41:e109646 (2022). PubMed: 35466426
- Carosi JM et al. Retromer regulates the lysosomal clearance of MAPT/tau. Autophagy 17:2217-2237 (2021). PubMed: 32960680
- González-Mancha N et al. Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse. Front Immunol 12:814570 (2021). PubMed: 35095913
- Halff EF et al. SNX27-Mediated Recycling of Neuroligin-2 Regulates Inhibitory Signaling. Cell Rep 29:2599-2607.e6 (2019). PubMed: 31775031
- Sapmaz A et al. USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7. Nat Commun 10:1454 (2019). PubMed: 30926795