Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293F and HeLa)
Loading amount
50 µg
Specification
HEK293F and HeLa
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA in TBS-Tween-20 (0.05%)
Secondary antibody
Name
Non-Abcam antibody was used: Goat-anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
2 second(s)
Bands
Specific: 40 kDa
Positive control
HEK293T and HeLa cell lysates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 17 2012