Anti-VPS26 抗体 (ab23892)
Key features and details
- Rabbit polyclonal to VPS26
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VPS26 antibody
VPS26 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VPS26 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Human VPS26 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab24288) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23892の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Abcam recommends using milk as the blocking agent. |
| ICC/IF | (5) |
Use a concentration of 1 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Abcam recommends using milk as the blocking agent. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). -
配列類似性
Belongs to the VPS26 family. -
細胞内局在
Cytoplasm. Endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 9559 Human
- Entrez Gene: 30930 Mouse
- Entrez Gene: 361846 Rat
- Omim: 605506 Human
- SwissProt: O75436 Human
- SwissProt: P40336 Mouse
- SwissProt: Q6AY86 Rat
- Unigene: 499925 Human
see all -
別名
- FLJ12930 antibody
- HB 58 antibody
- HB58 antibody
see all
画像
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Western blot - Anti-VPS26 antibody (ab23892)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
Lane 3: Jurkat cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab23892 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab23892 was shown to recognize VPS26 when VPS26 knockout samples were used, along with additional cross-reactive bands. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab23892 at a concentration of 1 µg/ml and ab8245 (loading control to GAPDH) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Western blot - Anti-VPS26 antibody (ab23892)All lanes : Anti-VPS26 antibody (ab23892) at 1 µg/ml
Lane 1 : Human kidney lysate
Lane 2 : Human kidney lysate with Human VPS26 peptide (ab24288) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 50 kDa. We are unsure as to the identity of these extra bands.ab23892 detects a band of ~ 38kDa in Human kidney lysate. This band is quenched by the addition of the immunizing peptide, ab24288. ab23892 also detects a band of the correct size in HEK293 and mouse kidney lysate (data not shown).
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Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)ICC/IF image of ab23892 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23892, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HepG2 cells.
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Western blot - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous AbreviewAll lanes : Anti-VPS26 antibody (ab23892) at 1/1000 dilution
Lane 1 : Rat NRK whole cell lysate at 25 µg
Lane 2 : Rat NRK whole cell lysate at 50 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 10 seconds
Blocked with 5% milk for 30 min at 25°C -
Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)Image from Xia B et al., PLoS One. 2010 Jul 23;5(7):e11771. Fig 3.; doi:10.1371/journal.pone.0011771; July 23, 2010, PLoS ONE 5(7): e11771.Immunofluorescence analysis of HeLa cells treated with mDpy-30 siRNA, staining VPS26 (green) with ab23892.
Cells were fixed formaldehyde, permeabilized and blocked with 5% goat serum for 20 minutes. Samples were incubated with primary antibody in blocking buffer for 2 hours before incubating with fluorophore-conjugated secondary antibody for 1 hour. Nuclei were detected using DAPI. -
Western blot - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous AbreviewAll lanes : Anti-VPS26 antibody (ab23892) at 1/1000 dilution
Lane 1 : HEK293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 2 seconds
Blocked with 5% milk for 30 min at 25°C -
Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous Abreview.Immunocytochemical analysis of Rat NRK cell line, labeling VPS26 with ab23892. Cells were formaldehyde fixed, permeabilized with 0.1% Triton X-100 in PBS for 3 minutes and blocked with 1% BSA for 30 minutes at 25°C. Cells were incubated with ab23892 diluted 1/250 in 1% BSA in PBS for 1 hour at 25°C.
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Western blot - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous Abreview.Anti-VPS26 antibody (ab23892) at 1/1000 dilution + COS whole cell lysate, African Green Monkey at 50 µg with Milk, 30 minutes, 25°C at 5 %
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (53)
ab23892 は 53 報の論文で使用されています。
- Niu M et al. VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease. Aging Cell 20:e13347 (2021). PubMed: 33745227
- Yasa S et al. CLN3 regulates endosomal function by modulating Rab7A-effector interactions. J Cell Sci 133:N/A (2020). PubMed: 32034082
- Rahman AA et al. Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N. Neuroscience 441:33-45 (2020). PubMed: 32540366
- Sharma P et al. SNX27-retromer assembly recycles MT1-MMP to invadopodia and promotes breast cancer metastasis. J Cell Biol 219:N/A (2020). PubMed: 31820782
- Xie J et al. TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry. Cell Rep 31:107750 (2020). PubMed: 32521275