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  1. Link

    vps26-antibody-ab23892.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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遺伝子ノックアウト細胞株 検証済

Anti-VPS26 抗体 (ab23892)

  • Datasheet
  • SDS
Reviews (12) Submit a question References (53)

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Western blot - Anti-VPS26 antibody (ab23892)
  • Western blot - Anti-VPS26 antibody (ab23892)
  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
  • Western blot - Anti-VPS26 antibody (ab23892)
  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
  • Western blot - Anti-VPS26 antibody (ab23892)
  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
  • Western blot - Anti-VPS26 antibody (ab23892)

Key features and details

  • Rabbit polyclonal to VPS26
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-VPS26 antibody [EPR13456] (ab181352)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-VPS26 antibody
    VPS26 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to VPS26
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to Human VPS26 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24288)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human VPS26 protein (ab130047)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab23892の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (5)
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Abcam recommends using milk as the blocking agent.

ICC/IF (5)
Use a concentration of 1 µg/ml.
特記事項
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

Abcam recommends using milk as the blocking agent.

ICC/IF
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA).
  • 配列類似性

    Belongs to the VPS26 family.
  • 細胞内局在

    Cytoplasm. Endosome membrane.
  • Target information above from: UniProt accession O75436 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 9559 Human
    • Entrez Gene: 30930 Mouse
    • Entrez Gene: 361846 Rat
    • Omim: 605506 Human
    • SwissProt: O75436 Human
    • SwissProt: P40336 Mouse
    • SwissProt: Q6AY86 Rat
    • Unigene: 499925 Human
    • Unigene: 260703 Mouse
    • Unigene: 473468 Mouse
    • Unigene: 3673 Rat
    see all
  • 別名

    • FLJ12930 antibody
    • HB 58 antibody
    • HB58 antibody
    • Hbeta 58 antibody
    • Hbeta 58 homolog antibody
    • Hbeta58 antibody
    • Hbeta58 homolog antibody
    • hVPS 26 antibody
    • hVPS26 antibody
    • MGC94168 antibody
    • PEP 8A antibody
    • PEP8A antibody
    • Vacuolar protein sorting 26 A antibody
    • Vacuolar protein sorting 26 antibody
    • Vacuolar protein sorting 26 homolog A antibody
    • Vacuolar protein sorting 26 homolog A S. pombe antibody
    • Vacuolar protein sorting 26 homolog A yeast antibody
    • Vacuolar protein sorting 26 yeast antibody
    • Vacuolar protein sorting 26 yeast homolog antibody
    • Vacuolar protein sorting 26A antibody
    • Vacuolar protein sorting associated protein 26A antibody
    • Vacuolar protein sorting-associated protein 26A antibody
    • Vesicle protein sorting 26 antibody
    • Vesicle protein sorting 26A antibody
    • VP26A_HUMAN antibody
    • VPS 26 antibody
    • VPS 26A antibody
    • VPS26A antibody
    see all

画像

  • Western blot - Anti-VPS26 antibody (ab23892)
    Western blot - Anti-VPS26 antibody (ab23892)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: VPS26 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lanes 1 - 3: Merged signal (red and green). Green - ab23892 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab23892 was shown to recognize VPS26 when VPS26 knockout samples were used, along with additional cross-reactive bands. Wild-type and VPS26 knockout samples were subjected to SDS-PAGE. ab23892 at a concentration of 1 µg/ml and ab8245 (loading control to GAPDH) diluted to 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-VPS26 antibody (ab23892)
    Western blot - Anti-VPS26 antibody (ab23892)
    All lanes : Anti-VPS26 antibody (ab23892) at 1 µg/ml

    Lane 1 : Human kidney lysate
    Lane 2 : Human kidney lysate with Human VPS26 peptide (ab24288) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 37 kDa why is the actual band size different from the predicted?
    Additional bands at: 25 kDa, 50 kDa. We are unsure as to the identity of these extra bands.



    ab23892 detects a band of ~ 38kDa in Human kidney lysate. This band is quenched by the addition of the immunizing peptide, ab24288. ab23892 also detects a band of the correct size in HEK293 and mouse kidney lysate (data not shown).

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)

    ICC/IF image of ab23892 stained human HEK 293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab23892, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HepG2 cells.

  • Western blot - Anti-VPS26 antibody (ab23892)
    Western blot - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous Abreview
    All lanes : Anti-VPS26 antibody (ab23892) at 1/1000 dilution

    Lane 1 : Rat NRK whole cell lysate at 25 µg
    Lane 2 : Rat NRK whole cell lysate at 50 µg

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 10 seconds


    Blocked with 5% milk for 30 min at 25°C

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)Image from Xia B et al., PLoS One. 2010 Jul 23;5(7):e11771. Fig 3.; doi:10.1371/journal.pone.0011771; July 23, 2010, PLoS ONE 5(7): e11771.
    Immunofluorescence analysis of HeLa cells treated with mDpy-30 siRNA, staining VPS26 (green) with ab23892.

    Cells were fixed formaldehyde, permeabilized and blocked with 5% goat serum for 20 minutes. Samples were incubated with primary antibody in blocking buffer for 2 hours before incubating with fluorophore-conjugated secondary antibody for 1 hour. Nuclei were detected using DAPI.
  • Western blot - Anti-VPS26 antibody (ab23892)
    Western blot - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous Abreview
    All lanes : Anti-VPS26 antibody (ab23892) at 1/1000 dilution

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 2 seconds


    Blocked with 5% milk for 30 min at 25°C

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)
    Immunocytochemistry/ Immunofluorescence - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous Abreview.

    Immunocytochemical analysis of Rat NRK cell line, labeling VPS26 with ab23892. Cells were formaldehyde fixed, permeabilized with 0.1% Triton X-100 in PBS for 3 minutes and blocked with 1% BSA for 30 minutes at 25°C. Cells were incubated with ab23892 diluted 1/250 in 1% BSA in PBS for 1 hour at 25°C.

    See Abreview

  • Western blot - Anti-VPS26 antibody (ab23892)
    Western blot - Anti-VPS26 antibody (ab23892)This image is courtesy of an anonymous Abreview.
    Anti-VPS26 antibody (ab23892) at 1/1000 dilution + COS whole cell lysate, African Green Monkey at 50 µg with Milk, 30 minutes, 25°C at 5 %

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 40 kDa


    Exposure time: 10 seconds

    See Abreview

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (53)

ab23892 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab23892 は 53 報の論文で使用されています。

  • Niu M  et al. VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease. Aging Cell 20:e13347 (2021). PubMed: 33745227
  • Yasa S  et al. CLN3 regulates endosomal function by modulating Rab7A-effector interactions. J Cell Sci 133:N/A (2020). PubMed: 32034082
  • Rahman AA  et al. Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N. Neuroscience 441:33-45 (2020). PubMed: 32540366
  • Sharma P  et al. SNX27-retromer assembly recycles MT1-MMP to invadopodia and promotes breast cancer metastasis. J Cell Biol 219:N/A (2020). PubMed: 31820782
  • Xie J  et al. TBC1D5-Catalyzed Cycling of Rab7 Is Required for Retromer-Mediated Human Papillomavirus Trafficking during Virus Entry. Cell Rep 31:107750 (2020). PubMed: 32521275
View all Publications for this product

レビューと Q&A

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1-10 of 12 Abreviews or Q&A

Western blot abreview for Anti-VPS26 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hek193T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Hek193T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jul 16 2020

Immunoprecipitation abreview for Anti-VPS26 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (CHRF)
Total protein in input
500 µg
Immuno-precipitation step
Protein A/G
Specification
CHRF
Read More

Abcam user community

Verified customer

投稿 Sep 14 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-VPS26 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (COS)
Specification
COS
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 28 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-VPS26 antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 26 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-VPS26 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (3T3)
Specification
3T3
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 26 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-VPS26 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (NRK)
Specification
NRK
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 26 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-VPS26 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Chinese Hamster Cell (CHO)
Specification
CHO
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS for 3 min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 26 2012

Western blot abreview for Anti-VPS26 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293F and HeLa)
Loading amount
50 µg
Specification
HEK293F and HeLa
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 17 2012

Western blot abreview for Anti-VPS26 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3)
Loading amount
75 µg
Specification
3T3
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 17 2012

Western blot abreview for Anti-VPS26 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (NRK)
Loading amount
25 µg
Specification
NRK
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Sep 17 2012

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