Anti-VPS15 抗体 [EPR5302(2)] - BSA and Azide free (ab248210)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5302(2)] to VPS15 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free
VPS15 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5302(2)] to VPS15 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Raji, C6, and Neuro-2a whole cell lysates. Mouse 14.5 brain, Rat E14 brain, and Rat P0 brain lysates.
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特記事項
ab248210 is the carrier-free version of ab128903.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.22 x 10 -10 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5302(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248210の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).
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特記事項 |
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WB
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa). |
ターゲット情報
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機能
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats. -
翻訳後修飾
Myristoylated.
Probably autophosphorylated. - Information by UniProt
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参照データベース
- Entrez Gene: 30849 Human
- Entrez Gene: 75669 Mouse
- Entrez Gene: 363131 Rat
- Omim: 602610 Human
- SwissProt: Q99570 Human
- SwissProt: Q8VD65 Mouse
- SwissProt: P0C0R5 Rat
- Unigene: 149032 Human
see all -
別名
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
see all
画像
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Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This antibody detects high background.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128903).
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All lanes : Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/500 dilution (Purified)
Lane 1 : Mouse E14.5 brain lysates
Lane 2 : Rat E14 brain lysates
Lane 3 : Rat P0 brain lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This antibody detects high background.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128903).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248210 は論文での使用が確認できていません。