Anti-VP16 tag 抗体 (ab4808)

Reviews (3)Q&A (4)References (22)

Key features and details

  • Rabbit polyclonal to VP16 tag
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-VP16 tag antibody

  • 製品の詳細

    Rabbit polyclonal to VP16 tag

  • 由来種

    Rabbit

  • 特異性

    This antibody was raised against an immunogen that is predicted to react with HSV-1. We do not expect this product to react with HSV-2. Abcam welcomes customer feedback and would appreciate any comments regarding this product.

  • アプリケーション

    適用あり: WB, IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide corresponding to Herpes simplex virus VP16 tag aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab13406)

  • ポジティブ･コントロール

    • This antibody recognises GAL4-VP16 recombinant fusion protein (30kDa) in Western blot.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 30 kDa.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (ab4808)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VP16 tag antibody (ab4808)

    ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488 ® conjugated Donkey anti-Rabbit IgG.

    This image is courtesy of an Abreview submitted on 10 March 2006.

    See Abreview

  • Western blot - Anti-VP16 tag antibody (ab4808)
    Western blot - Anti-VP16 tag antibody (ab4808)
    Lane 1 : Marker
    Lanes 2-3 : Anti-VP16 tag antibody (ab4808) at 1 µg/ml

    Lane 2 : GAL4-VP16 human recombinant E.coli

    Lane 3 : GAL4-VP16 human recombinant E.coli
    with VP16 tag peptide (ab13406) at 1 µg/ml

    Secondary
    Lanes 2-3 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

    Performed under reducing conditions.

参考文献 (22)

ab4808 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4808 は 22 報の論文で使用されています。

  • Rodrigue A  et al. A global functional analysis of missense mutations reveals two major hotspots in the PALB2 tumor suppressor. Nucleic Acids Res 47:10662-10677 (2019). PubMed: 31586400
  • Saito AN  et al. Structure-function study of a novel inhibitor of the casein kinase 1 family in Arabidopsis thaliana. Plant Direct 3:e00172 (2019). PubMed: 31549020
  • Wang L  et al. Two Paralogous Tetraspanins TSP-12 and TSP-14 Function with the ADAM10 Metalloprotease SUP-17 to Promote BMP Signaling in Caenorhabditis elegans. PLoS Genet 13:e1006568 (2017). PubMed: 28068334
  • Fehrenbacher N  et al. The G protein-coupled receptor GPR31 promotes membrane association of KRAS. J Cell Biol 216:2329-2338 (2017). PubMed: 28619714
  • Smith RWP  et al. Viral and cellular mRNA-specific activators harness PABP and eIF4G to promote translation initiation downstream of cap binding. Proc Natl Acad Sci U S A 114:6310-6315 (2017). PubMed: 28559344
View all Publications for this product

レビューと Q&A

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1-7 of 7 Abreviews or Q&A

Question

Dear Abcam, I am potentially interested in purchasing the anti-VP16 tag antibody (ab4808). However, it is not completely clear to me whether I would be able to detect rtTA with this particular antibody. Could you inform me on this? Thank you very much in advance. Sincerely,

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Answer

Thank you for your inquiry. Unfortunately, I am sorry this antibody has not been tested for detection of the endogenous full length tetracycline reverse transcriptional activator protein. It is quite likely to detect, as the VP16 tag is from the C terminus of this protein as far as I am aware. However, we would not be able to guarantee how well it would work for detection of rtTA. Regrettably, we have no other antibodies in the catalogue raised against rtTA. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com http://www.biocompare.com/ProductCategories/2045/Antibodies.html I am sorry we do not have an antibody tested for your requirements on this occasion. However, I hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions.

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Immunohistochemistry (Frozen sections) abreview for Anti-VP16 tag antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Rat brain)
Specification
Rat brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Apr 07 2009

Western blot abreview for Anti-VP16 tag antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Hamster Cell lysate - whole cell (chinese hamster ovaries)
Loading amount
20 µg
Specification
chinese hamster ovaries
Treatment
cells were overexpressing a VP16 fusion protein
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Apr 02 2008

Question

BATCH NUMBER 116137 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal and non specific staining specific staining should be in the hippocampus, and there is no staining in my specimen. non-specific staining appear in purkinje cells in the cerebellum in vp-16 negative and positive brains. SAMPLE brains of transgenic mice camkII-tTa. VP16 is a part of the tTa element. DETECTION METHOD AEC POSITIVE AND NEGATIVE CONTROLS USED brains of non transgenic mice as negative control. The positive brains were positive in genotyping as well as in the phenotype. ANTIBODY STORAGE CONDITIONS 4 degrees FIXATION OF SAMPLE formalin-fixed paraffin embedded ANTIGEN RETRIEVAL citrate ph6 - microwave PERMEABILIZATION STEP non BLOCKING CONDITIONS 1% BSA in PBS SECONDARY ANTIBODY vector - universal anti mouse+rabbit HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2-3 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? different antigen retrieval protocols: different buffers in the heating antigen retrieval (EDTA, Glycine) as well as pronase (1mg/ml) enzymatic treatmant.

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Answer

Thank you for your enquiry. I am sorry to hear that your customer has not been able to obtain satisfactory results using this antibody. I appreciate their frustration. I am assuming that your customer is using a transgenic mouse with a camkII-tTa-VP16 construct. We have received no complaints about this antibody to date. We have also received positive feedback from a customer that applied this antibody following paraformaldehyde fixation using an overnight incubation. My concern with the approach that your customer is using this antibody without permeabilization; something that is employed by the reviewer that I would consider necessary for use with this antibody. I would like to recommend the use of 0.1% Triton for this purpose. They also do not mention the dilution that they have been applying this antibody at. This antibody has been shown to give good results at a dilution of 1/100. I would recommend an overnight incubation at 4oC. It may also help if your cusomer reduces the dilution of the secondary antibody that they have been using. This combined with a primary antibody dilution of 1/100 can only serve to improve the results that you have been obtaining. Finally I would like to ask for clarification over the CamKII construct that they have been targeting. This antibody targets the VP16 activation domain within amino acids 413-490; can you tell me whether this protein construct contains full length VP16? I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Is the epitope of the antibody located within the activation domain of VP16?

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Answer

Thank you for your enquiry. The VP16 activation domain is amino acids 413-490. The peptide that this antibody was raised to lies within this region. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VP16 tag antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
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Abcam user community

Verified customer

投稿 Mar 10 2006

Question

Does this antibody recognize both tags, gal4 and vp16 alone, or does it only recognize the fusionprotein??

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Answer

Thank you for your enquiry. This antibody recognizes VP16 only. The immunogen was derived from a synthetic peptide derived from residues 450 - 550 of HSV VP16 tag. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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