製品の概要

  • 製品名
    Anti-Von Willebrand Factor antibody [F8/86]
    Von Willebrand Factor 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [F8/86] to Von Willebrand Factor
  • 由来種
    Mouse
  • アプリケーション
    適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit
  • 免疫原

    Von Willebrand Factor isolated from human plasma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/15 - 1/30. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/15 - 1/30. ABC method. We suggest an incubation period of 60 minutes at room temperature.

ターゲット情報

  • 機能
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 組織特異性
    Plasma.
  • 関連疾患
    Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • 配列類似性
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • ドメイン
    The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • 翻訳後修飾
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • 細胞内局在
    Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

画像

  • Formalin fixed paraffin embedded human tonsil stained with Von Willebrand Factor using ABC and AEC chromogen.

プロトコール

参考文献

This product has been referenced in:
  • Zhang W  et al. Cell-free therapy based on adipose tissue stem cell-derived exosomes promotes wound healing via the PI3K/Akt signaling pathway. Exp Cell Res 370:333-342 (2018). Read more (PubMed: 29964051) »
  • Wang Y  et al. The transplantation of Akt-overexpressing amniotic fluid-derived mesenchymal stem cells protects the heart against ischemia-reperfusion injury in rabbits. Mol Med Rep 14:234-42 (2016). IHC-P ; Rabbit . Read more (PubMed: 27151366) »
See all 8 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Sample
Rabbit Tissue sections (trachea)
Specification
trachea
Permeabilization
Yes - triton X-100
Fixative
Paraformaldehyde

Ms. Hee-Bok Kim

Verified customer

投稿 Jun 05 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
1% BSA + 5% NGS + 0.1% Sapo as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
Antigen retrieval step
None
Sample
Human Tissue sections (blood vessels)
Specification
blood vessels
Permeabilization
Yes - saponine
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Mar 24 2014

Answer

Thank you for contacting us.

The lab sent me the following information regarding your question:

Based on the data Factor VIII reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels and shows finely granular cytoplasmic staining. It also reacts with endocardium, platelets, and megakaryocytes.

Please let me know if this information is sufficient.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

I have emailed the lab to check regarding the specificity testing, and I'll let you know what I find out.
The IHC image on the datasheet is from the lab.

The immunogen used to raise the antibody was Von Willebrand Factor isolated from human plasma. We carry vWF protein (ab88533 and ab88555) that can be used for blocking, although we have not tried that application with these 2 proteins.

https://www.abcam.com/Von-Willebrand-Factor-protein-Human-ab88533.html
https://www.abcam.com/Von-Willebrand-Factor-protein-Human-ab88555.html

You can use our testing discount program to try either protein in a blocking experiment.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the antibody/protein/kit at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody/protein at the full price (100%) of the antibody/protein you have tested, or a full price discount for the amount paid for the kit. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
This programapplies to ELISA kits and other kits we offer.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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